Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22502 | 5' | -54.3 | NC_005069.1 | + | 42369 | 0.66 | 0.759989 |
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Target: 5'- aGCCGGuuacGGuuCCGGCgu-CACACCuACCa -3' miRNA: 3'- -CGGCCc---UC--GGCUGaauGUGUGGuUGG- -5' |
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| 22502 | 5' | -54.3 | NC_005069.1 | + | 30864 | 0.67 | 0.70679 |
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Target: 5'- -aUGGGGGCUGGgcCUgACGCugCAGCUc -3' miRNA: 3'- cgGCCCUCGGCU--GAaUGUGugGUUGG- -5' |
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| 22502 | 5' | -54.3 | NC_005069.1 | + | 8359 | 0.67 | 0.67388 |
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Target: 5'- aGCUGGGAGCaUGGCguauucugGCGaggauuuuCCAGCCa -3' miRNA: 3'- -CGGCCCUCG-GCUGaa------UGUgu------GGUUGG- -5' |
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| 22502 | 5' | -54.3 | NC_005069.1 | + | 15790 | 0.67 | 0.651719 |
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Target: 5'- uGCgGGuGAaCCGACUuauauUGCugGCCAGCa -3' miRNA: 3'- -CGgCC-CUcGGCUGA-----AUGugUGGUUGg -5' |
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| 22502 | 5' | -54.3 | NC_005069.1 | + | 22199 | 0.68 | 0.596176 |
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Target: 5'- aGCUGGuuGCCGAgUUACAgaGCCAGgCg -3' miRNA: 3'- -CGGCCcuCGGCUgAAUGUg-UGGUUgG- -5' |
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| 22502 | 5' | -54.3 | NC_005069.1 | + | 45165 | 0.73 | 0.363896 |
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Target: 5'- uGUCGGGAcugccCCGGCUUgugGCGCuuCCAGCCa -3' miRNA: 3'- -CGGCCCUc----GGCUGAA---UGUGu-GGUUGG- -5' |
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| 22502 | 5' | -54.3 | NC_005069.1 | + | 45866 | 0.73 | 0.330259 |
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Target: 5'- uGUCGGGAcugccCCGGCUUAUGCcgcuuCCAGCCa -3' miRNA: 3'- -CGGCCCUc----GGCUGAAUGUGu----GGUUGG- -5' |
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| 22502 | 5' | -54.3 | NC_005069.1 | + | 42600 | 1.15 | 0.000419 |
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Target: 5'- cGCCGGGAGCCGACUUACACACCAACCg -3' miRNA: 3'- -CGGCCCUCGGCUGAAUGUGUGGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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