miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22512 5' -54.1 NC_005074.1 + 2420 0.68 0.113493
Target:  5'- -gUGCAGCUAUCUcgccUGCAC-GCUGCa -3'
miRNA:   3'- ugGCGUCGAUAGGaa--AUGUGcCGGCG- -5'
22512 5' -54.1 NC_005074.1 + 883 0.68 0.112333
Target:  5'- gAUCGCGGCUgggcaaggagauuuGUUUgggUAUGCGGUCGCa -3'
miRNA:   3'- -UGGCGUCGA--------------UAGGaa-AUGUGCCGGCG- -5'
22512 5' -54.1 NC_005074.1 + 2558 0.74 0.035628
Target:  5'- cCCGCAGCUugcagggcugcGUCCUgUUGCAUGGCUucaGCc -3'
miRNA:   3'- uGGCGUCGA-----------UAGGA-AAUGUGCCGG---CG- -5'
22512 5' -54.1 NC_005074.1 + 3114 1.12 0.000018
Target:  5'- aACCGCAGCUAUCCUUUACACGGCCGCg -3'
miRNA:   3'- -UGGCGUCGAUAGGAAAUGUGCCGGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.