Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22512 | 5' | -54.1 | NC_005074.1 | + | 3114 | 1.12 | 1.8e-05 |
Target: 5'- aACCGCAGCUAUCCUUUACACGGCCGCg -3' miRNA: 3'- -UGGCGUCGAUAGGAAAUGUGCCGGCG- -5' |
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22512 | 5' | -54.1 | NC_005074.1 | + | 883 | 0.68 | 0.112333 |
Target: 5'- gAUCGCGGCUgggcaaggagauuuGUUUgggUAUGCGGUCGCa -3' miRNA: 3'- -UGGCGUCGA--------------UAGGaa-AUGUGCCGGCG- -5' |
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22512 | 5' | -54.1 | NC_005074.1 | + | 2558 | 0.74 | 0.035628 |
Target: 5'- cCCGCAGCUugcagggcugcGUCCUgUUGCAUGGCUucaGCc -3' miRNA: 3'- uGGCGUCGA-----------UAGGA-AAUGUGCCGG---CG- -5' |
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22512 | 5' | -54.1 | NC_005074.1 | + | 2420 | 0.68 | 0.113493 |
Target: 5'- -gUGCAGCUAUCUcgccUGCAC-GCUGCa -3' miRNA: 3'- ugGCGUCGAUAGGaa--AUGUGcCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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