Results 1 - 11 of 11 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2252 | 3' | -58.8 | NC_001405.1 | + | 25945 | 0.66 | 0.335038 |
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Target: 5'- --aCCCCGgAGCCGCGGACUUUuCg- -3' miRNA: 3'- aaaGGGGCgUUGGCGUCUGGGAcGau -5' |
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| 2252 | 3' | -58.8 | NC_001405.1 | + | 18592 | 0.66 | 0.326725 |
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Target: 5'- ---aCCCGCccuAGCCGCGcGuCCCUGCg- -3' miRNA: 3'- aaagGGGCG---UUGGCGU-CuGGGACGau -5' |
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| 2252 | 3' | -58.8 | NC_001405.1 | + | 16205 | 0.66 | 0.326725 |
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Target: 5'- --gCCCaacgCGCGGCgGCGG-CCCUGCUu -3' miRNA: 3'- aaaGGG----GCGUUGgCGUCuGGGACGAu -5' |
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| 2252 | 3' | -58.8 | NC_001405.1 | + | 12802 | 0.67 | 0.280076 |
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Target: 5'- -gUCCCCGCGGCacguugGCGGGCUgUGUa- -3' miRNA: 3'- aaAGGGGCGUUGg-----CGUCUGGgACGau -5' |
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| 2252 | 3' | -58.8 | NC_001405.1 | + | 11179 | 0.68 | 0.272836 |
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Target: 5'- --cCCCCGCGGCgC-CGGGCCCgGCa- -3' miRNA: 3'- aaaGGGGCGUUG-GcGUCUGGGaCGau -5' |
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| 2252 | 3' | -58.8 | NC_001405.1 | + | 12942 | 0.68 | 0.258808 |
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Target: 5'- -cUCCCacagGCGACCGCGcGACCgUGuCUAg -3' miRNA: 3'- aaAGGGg---CGUUGGCGU-CUGGgAC-GAU- -5' |
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| 2252 | 3' | -58.8 | NC_001405.1 | + | 12427 | 0.68 | 0.258808 |
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Target: 5'- gUUCCggcaGCAGCCGCAGGCCaaccgGCUc -3' miRNA: 3'- aAAGGgg--CGUUGGCGUCUGGga---CGAu -5' |
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| 2252 | 3' | -58.8 | NC_001405.1 | + | 5581 | 0.69 | 0.226315 |
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Target: 5'- ---aUCCGC-GCCGCAGGCCCcGCa- -3' miRNA: 3'- aaagGGGCGuUGGCGUCUGGGaCGau -5' |
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| 2252 | 3' | -58.8 | NC_001405.1 | + | 3348 | 0.73 | 0.111695 |
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Target: 5'- ---aCCCGCAccaGgUGCAGACCCUGCg- -3' miRNA: 3'- aaagGGGCGU---UgGCGUCUGGGACGau -5' |
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| 2252 | 3' | -58.8 | NC_001405.1 | + | 26178 | 0.75 | 0.078472 |
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Target: 5'- --gCCCUaucaGCAGCCGCGGGCCCUuGCUu -3' miRNA: 3'- aaaGGGG----CGUUGGCGUCUGGGA-CGAu -5' |
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| 2252 | 3' | -58.8 | NC_001405.1 | + | 13552 | 1.04 | 0.0004 |
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Target: 5'- uUUUCCCCGCAACCGCAGACCCUGCUAg -3' miRNA: 3'- -AAAGGGGCGUUGGCGUCUGGGACGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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