Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22524 | 5' | -45.1 | NC_005083.1 | + | 28297 | 0.66 | 0.999999 |
Target: 5'- uUGAAUAUGCUU--CUUGAGCAGUa- -3' miRNA: 3'- uGCUUGUACGGAcuGAACUUGUUAaa -5' |
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22524 | 5' | -45.1 | NC_005083.1 | + | 16558 | 0.66 | 0.999999 |
Target: 5'- cAUGAACAacugGCUUGGCUUGuGAUAGUa- -3' miRNA: 3'- -UGCUUGUa---CGGACUGAAC-UUGUUAaa -5' |
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22524 | 5' | -45.1 | NC_005083.1 | + | 134439 | 0.66 | 0.999999 |
Target: 5'- uUGAACAUGCUUGAUUUcAAUAAa-- -3' miRNA: 3'- uGCUUGUACGGACUGAAcUUGUUaaa -5' |
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22524 | 5' | -45.1 | NC_005083.1 | + | 84432 | 0.66 | 0.999999 |
Target: 5'- -aGAAaAUGCCUGACcuUUGAACGu--- -3' miRNA: 3'- ugCUUgUACGGACUG--AACUUGUuaaa -5' |
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22524 | 5' | -45.1 | NC_005083.1 | + | 168975 | 0.66 | 0.999999 |
Target: 5'- --uGACGUGUCUGGCUcuaUGAGCAc--- -3' miRNA: 3'- ugcUUGUACGGACUGA---ACUUGUuaaa -5' |
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22524 | 5' | -45.1 | NC_005083.1 | + | 206004 | 0.67 | 0.999996 |
Target: 5'- uCGAACc-GCCUGuaccaccuGCUUGAACAAUg- -3' miRNA: 3'- uGCUUGuaCGGAC--------UGAACUUGUUAaa -5' |
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22524 | 5' | -45.1 | NC_005083.1 | + | 81329 | 0.67 | 0.999995 |
Target: 5'- cAUGAGCGUGCUucauguuguUGACUUGccgGACGGUa- -3' miRNA: 3'- -UGCUUGUACGG---------ACUGAAC---UUGUUAaa -5' |
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22524 | 5' | -45.1 | NC_005083.1 | + | 84850 | 0.69 | 0.999948 |
Target: 5'- cACGA--GUGCCUGGCUUGuuGCAc--- -3' miRNA: 3'- -UGCUugUACGGACUGAACu-UGUuaaa -5' |
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22524 | 5' | -45.1 | NC_005083.1 | + | 126851 | 0.7 | 0.999798 |
Target: 5'- gACGGugAgaaacUGCCUGACUuUGAGCAc--- -3' miRNA: 3'- -UGCUugU-----ACGGACUGA-ACUUGUuaaa -5' |
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22524 | 5' | -45.1 | NC_005083.1 | + | 147052 | 0.7 | 0.999741 |
Target: 5'- cGCGcuCAUGCUUGACUUGAGu----- -3' miRNA: 3'- -UGCuuGUACGGACUGAACUUguuaaa -5' |
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22524 | 5' | -45.1 | NC_005083.1 | + | 39508 | 1.07 | 0.059418 |
Target: 5'- cACGAACAUGCCUGACUUGAACAAUUUa -3' miRNA: 3'- -UGCUUGUACGGACUGAACUUGUUAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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