Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22525 | 5' | -45 | NC_005083.1 | + | 23760 | 0.66 | 1 |
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Target: 5'- aGUGUA-AUUGUCAACGUCgUAUUUUu -3' miRNA: 3'- -UACGUgUGACAGUUGCAGaAUAAAGu -5' |
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| 22525 | 5' | -45 | NC_005083.1 | + | 185222 | 0.66 | 1 |
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Target: 5'- cUGCGCcuuCUGguaCAGCGUCUUugauguUUUCAc -3' miRNA: 3'- uACGUGu--GACa--GUUGCAGAAu-----AAAGU- -5' |
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| 22525 | 5' | -45 | NC_005083.1 | + | 203404 | 0.66 | 1 |
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Target: 5'- aGUGCugACgUGUCAAUGUCa------- -3' miRNA: 3'- -UACGugUG-ACAGUUGCAGaauaaagu -5' |
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| 22525 | 5' | -45 | NC_005083.1 | + | 89689 | 0.66 | 1 |
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Target: 5'- -aGCACACUGaacgCAGgGUCU---UUCAa -3' miRNA: 3'- uaCGUGUGACa---GUUgCAGAauaAAGU- -5' |
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| 22525 | 5' | -45 | NC_005083.1 | + | 153285 | 0.66 | 0.999999 |
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Target: 5'- uGUGCGCuCUGUCGA-GUCUUGa---- -3' miRNA: 3'- -UACGUGuGACAGUUgCAGAAUaaagu -5' |
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| 22525 | 5' | -45 | NC_005083.1 | + | 170099 | 0.67 | 0.999999 |
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Target: 5'- cAUGCACGCaguugaGUCGGgGUCUaGUUUCc -3' miRNA: 3'- -UACGUGUGa-----CAGUUgCAGAaUAAAGu -5' |
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| 22525 | 5' | -45 | NC_005083.1 | + | 40544 | 0.68 | 0.999993 |
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Target: 5'- -aGCACAUUGUC-ACGcUCUUuUUUCu -3' miRNA: 3'- uaCGUGUGACAGuUGC-AGAAuAAAGu -5' |
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| 22525 | 5' | -45 | NC_005083.1 | + | 211744 | 0.69 | 0.999966 |
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Target: 5'- gGUGCGCcuAUUGUCAACGUCg------- -3' miRNA: 3'- -UACGUG--UGACAGUUGCAGaauaaagu -5' |
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| 22525 | 5' | -45 | NC_005083.1 | + | 24114 | 0.69 | 0.999939 |
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Target: 5'- -gGCGCACUGUUAACGUaCUg------ -3' miRNA: 3'- uaCGUGUGACAGUUGCA-GAauaaagu -5' |
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| 22525 | 5' | -45 | NC_005083.1 | + | 52565 | 1.08 | 0.051151 |
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Target: 5'- cAUGCACACUGUCAACGUCUUAUUUCAa -3' miRNA: 3'- -UACGUGUGACAGUUGCAGAAUAAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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