miRNA: 3'- cgGAcCC-GAAC--UAUGCGAGCCUGAUg -5'
miRNA: 3'- -CGGACCCGaACU---AUGCGAG--------CCUGaug -5'
miRNA: 3'- -CGGACCCGAAcua-UGCgAGCCUGAUG- -5'
miRNA: 3'- -CGGACCCGaacUAUGCGAGCCuGAUg -5'
miRNA: 3'- -CGGACCCGAACUAUGCGAGCCUGAUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22526 | 5' | -54 | NC_005083.1 | + | 114198 | 0.68 | 0.959969 |
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Target: 5'- -aCUcGGuCUUGuuGUGCGCUCGGGCUGu -3' miRNA: 3'- cgGAcCC-GAAC--UAUGCGAGCCUGAUg -5' |
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| 22526 | 5' | -54 | NC_005083.1 | + | 95328 | 0.68 | 0.95246 |
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Target: 5'- uGCCUGGGU-UGAacuUACGCUCaacgagaaGGACa-- -3' miRNA: 3'- -CGGACCCGaACU---AUGCGAG--------CCUGaug -5' |
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| 22526 | 5' | -54 | NC_005083.1 | + | 14263 | 0.71 | 0.851965 |
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Target: 5'- uGCUUGGGUUUacucGCGgUUGGACUACa -3' miRNA: 3'- -CGGACCCGAAcua-UGCgAGCCUGAUG- -5' |
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| 22526 | 5' | -54 | NC_005083.1 | + | 201973 | 0.73 | 0.775113 |
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Target: 5'- cGUUUGGGCgc-GUGCGCUCGGuCUGa -3' miRNA: 3'- -CGGACCCGaacUAUGCGAGCCuGAUg -5' |
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| 22526 | 5' | -54 | NC_005083.1 | + | 56750 | 1.12 | 0.004303 |
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Target: 5'- uGCCUGGGCUUGAUACGCUCGGACUACg -3' miRNA: 3'- -CGGACCCGAACUAUGCGAGCCUGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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