Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22528 | 5' | -47.3 | NC_005083.1 | + | 35626 | 0.66 | 0.999987 |
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Target: 5'- aGUUUCAGcacGCGGuCAAGcUGcUCGUGAcGCa -3' miRNA: 3'- -CAAAGUC---CGUC-GUUCuAC-AGCAUU-CG- -5' |
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| 22528 | 5' | -47.3 | NC_005083.1 | + | 75599 | 0.66 | 0.999975 |
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Target: 5'- uGUUgagCAcGGUAauaugcGCAAGAaaUGUCGUGAGUu -3' miRNA: 3'- -CAAa--GU-CCGU------CGUUCU--ACAGCAUUCG- -5' |
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| 22528 | 5' | -47.3 | NC_005083.1 | + | 30656 | 0.67 | 0.999954 |
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Target: 5'- -cUUCAGGCAGUuuuuucAUGUUGUAcugauauguuGGCg -3' miRNA: 3'- caAAGUCCGUCGuuc---UACAGCAU----------UCG- -5' |
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| 22528 | 5' | -47.3 | NC_005083.1 | + | 77782 | 0.68 | 0.999824 |
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Target: 5'- ---aCAGGCaAGUuucuagauuGGGAUGUCGUGcucgaAGCg -3' miRNA: 3'- caaaGUCCG-UCG---------UUCUACAGCAU-----UCG- -5' |
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| 22528 | 5' | -47.3 | NC_005083.1 | + | 88053 | 0.69 | 0.9993 |
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Target: 5'- --aUCGGcGCAGCAguGGGUGgcuUUGUAGGUa -3' miRNA: 3'- caaAGUC-CGUCGU--UCUAC---AGCAUUCG- -5' |
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| 22528 | 5' | -47.3 | NC_005083.1 | + | 14446 | 0.69 | 0.999138 |
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Target: 5'- cUUUCAGuGCAGU--GAUGUUGUAaaaaAGCu -3' miRNA: 3'- cAAAGUC-CGUCGuuCUACAGCAU----UCG- -5' |
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| 22528 | 5' | -47.3 | NC_005083.1 | + | 190869 | 0.71 | 0.995645 |
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Target: 5'- ----aGGGCAGgAAGAUuaUCGUGAGCu -3' miRNA: 3'- caaagUCCGUCgUUCUAc-AGCAUUCG- -5' |
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| 22528 | 5' | -47.3 | NC_005083.1 | + | 53214 | 0.87 | 0.451805 |
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Target: 5'- ---gCAGGUAGCGGGAUGUCGUcaAAGCu -3' miRNA: 3'- caaaGUCCGUCGUUCUACAGCA--UUCG- -5' |
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| 22528 | 5' | -47.3 | NC_005083.1 | + | 78028 | 1.12 | 0.018939 |
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Target: 5'- uGUUUCAGGCAGCAAGAUGUCGUAAGCc -3' miRNA: 3'- -CAAAGUCCGUCGUUCUACAGCAUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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