Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2253 | 3' | -49.9 | NC_001405.1 | + | 13444 | 0.66 | 0.857562 |
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Target: 5'- -cGAUGCaAGUAGUCCAU---UAGGCg -3' miRNA: 3'- guCUACGcUCAUCGGGUAaagGUUCG- -5' |
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| 2253 | 3' | -49.9 | NC_001405.1 | + | 26959 | 0.66 | 0.857562 |
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Target: 5'- aCAGGUGgGAaaaauCCUAUUUCUAAGCu -3' miRNA: 3'- -GUCUACgCUcauc-GGGUAAAGGUUCG- -5' |
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| 2253 | 3' | -49.9 | NC_001405.1 | + | 16284 | 0.67 | 0.809473 |
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Target: 5'- uGGccGCGGGUAuugucacuguGCCCcccaggUCCAGGCg -3' miRNA: 3'- gUCuaCGCUCAU----------CGGGuaa---AGGUUCG- -5' |
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| 2253 | 3' | -49.9 | NC_001405.1 | + | 16976 | 0.68 | 0.733445 |
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Target: 5'- gUAGGUGuUGGGUuGCCCucgUCCAgcGGCa -3' miRNA: 3'- -GUCUAC-GCUCAuCGGGuaaAGGU--UCG- -5' |
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| 2253 | 3' | -49.9 | NC_001405.1 | + | 18553 | 0.71 | 0.592216 |
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Target: 5'- aCAGGUGUGuGcUGGCCCAgcacUCCGguAGCc -3' miRNA: 3'- -GUCUACGCuC-AUCGGGUaa--AGGU--UCG- -5' |
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| 2253 | 3' | -49.9 | NC_001405.1 | + | 16839 | 0.71 | 0.592216 |
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Target: 5'- gUAGGUGCGGGUGgagcGCUCA---CCGGGCg -3' miRNA: 3'- -GUCUACGCUCAU----CGGGUaaaGGUUCG- -5' |
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| 2253 | 3' | -49.9 | NC_001405.1 | + | 13675 | 1.12 | 0.001149 |
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Target: 5'- uCAGAUGCGAGUAGCCCAUUUCCAAGCu -3' miRNA: 3'- -GUCUACGCUCAUCGGGUAAAGGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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