Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2253 | 5' | -60.4 | NC_001405.1 | + | 23904 | 0.71 | 0.142615 |
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Target: 5'- -cGCUGGUGC-CGCGCaCACCCaGCCCGc -3' miRNA: 3'- aaUGGUCGUGaGCGUG-GUGGG-CGGGC- -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 12383 | 0.71 | 0.138685 |
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Target: 5'- -gGCUGGCuCUgcaGCGCCGCCCGCaCCGc -3' miRNA: 3'- aaUGGUCGuGAg--CGUGGUGGGCG-GGC- -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 16412 | 0.71 | 0.131123 |
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Target: 5'- -gGCCuGCGCgugccCGUGCgCACCCGCCCc -3' miRNA: 3'- aaUGGuCGUGa----GCGUG-GUGGGCGGGc -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 6695 | 0.72 | 0.12049 |
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Target: 5'- aUACgAGCg--CGCGCUugCCGCCCGc -3' miRNA: 3'- aAUGgUCGugaGCGUGGugGGCGGGC- -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 12085 | 0.73 | 0.104238 |
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Target: 5'- -cAUCAGCucgcggucgcugaGCUCGCGCCGCCgGCUCa -3' miRNA: 3'- aaUGGUCG-------------UGAGCGUGGUGGgCGGGc -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 12247 | 0.74 | 0.083081 |
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Target: 5'- gUUGCCAGCGCgCGCggguGCCACCgccaGCCCa -3' miRNA: 3'- -AAUGGUCGUGaGCG----UGGUGGg---CGGGc -5' |
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| 2253 | 5' | -60.4 | NC_001405.1 | + | 13714 | 1.05 | 0.000298 |
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Target: 5'- uUUACCAGCACUCGCACCACCCGCCCGc -3' miRNA: 3'- -AAUGGUCGUGAGCGUGGUGGGCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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