Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22530 | 3' | -43.4 | NC_005083.1 | + | 62618 | 0.67 | 1 |
Target: 5'- uUCAUGuAAUGCAGUgcuauacaaCUGUGguuuCUGCAAu -3' miRNA: 3'- -AGUAC-UUACGUUA---------GACAUu---GACGUUu -5' |
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22530 | 3' | -43.4 | NC_005083.1 | + | 221345 | 0.66 | 1 |
Target: 5'- cCAUGAcgGCAuUUUGUagucaGACUGCu-- -3' miRNA: 3'- aGUACUuaCGUuAGACA-----UUGACGuuu -5' |
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22530 | 3' | -43.4 | NC_005083.1 | + | 8408 | 0.68 | 1 |
Target: 5'- gUCGUGAAUGguGUCUGaccauuugaaguCUGCAu- -3' miRNA: 3'- -AGUACUUACguUAGACauu---------GACGUuu -5' |
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22530 | 3' | -43.4 | NC_005083.1 | + | 194956 | 0.69 | 0.999998 |
Target: 5'- uUCAcGAGUcGCAAUCUGUAGCagGuCAAGu -3' miRNA: 3'- -AGUaCUUA-CGUUAGACAUUGa-C-GUUU- -5' |
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22530 | 3' | -43.4 | NC_005083.1 | + | 169062 | 0.69 | 0.999998 |
Target: 5'- gUCAUGGuaAUGC-GUCUGUAGCgaacgaGCAAu -3' miRNA: 3'- -AGUACU--UACGuUAGACAUUGa-----CGUUu -5' |
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22530 | 3' | -43.4 | NC_005083.1 | + | 238460 | 0.69 | 0.999998 |
Target: 5'- aUCGUGAugaGgGAUCUGUAACUGa--- -3' miRNA: 3'- -AGUACUua-CgUUAGACAUUGACguuu -5' |
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22530 | 3' | -43.4 | NC_005083.1 | + | 56697 | 0.7 | 0.999983 |
Target: 5'- aUCAUGGG-GCAGggUUGUAACUGCu-- -3' miRNA: 3'- -AGUACUUaCGUUa-GACAUUGACGuuu -5' |
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22530 | 3' | -43.4 | NC_005083.1 | + | 36333 | 0.72 | 0.9997 |
Target: 5'- -gGUcGAUGuCAGUCUGUGACUGUAAc -3' miRNA: 3'- agUAcUUAC-GUUAGACAUUGACGUUu -5' |
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22530 | 3' | -43.4 | NC_005083.1 | + | 122747 | 0.75 | 0.996951 |
Target: 5'- aUCGUGcucgauuGAUGUAAUCUG-AACUGCAAu -3' miRNA: 3'- -AGUAC-------UUACGUUAGACaUUGACGUUu -5' |
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22530 | 3' | -43.4 | NC_005083.1 | + | 166450 | 0.78 | 0.98214 |
Target: 5'- aUCGUGAGUaugagagucgucaGCAA-CUGUAACUGCGAGu -3' miRNA: 3'- -AGUACUUA-------------CGUUaGACAUUGACGUUU- -5' |
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22530 | 3' | -43.4 | NC_005083.1 | + | 103514 | 1.09 | 0.063114 |
Target: 5'- aUCAUGAAUGCAAUCUGUAACUGCAAAg -3' miRNA: 3'- -AGUACUUACGUUAGACAUUGACGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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