Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22531 | 3' | -47.3 | NC_005083.1 | + | 164701 | 0.67 | 0.999895 |
Target: 5'- cAAUGacGUUCuUAACCAUcagguuaCCGCCAGUCg -3' miRNA: 3'- aUUACa-CGAG-AUUGGUA-------GGUGGUUAG- -5' |
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22531 | 3' | -47.3 | NC_005083.1 | + | 165400 | 0.69 | 0.999139 |
Target: 5'- aUAAUG-GgUCUAGCUAUUCACCGGg- -3' miRNA: 3'- -AUUACaCgAGAUUGGUAGGUGGUUag -5' |
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22531 | 3' | -47.3 | NC_005083.1 | + | 86617 | 0.73 | 0.975408 |
Target: 5'- --uUGUGCUCaUAGCCAUCCaaACC-GUCc -3' miRNA: 3'- auuACACGAG-AUUGGUAGG--UGGuUAG- -5' |
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22531 | 3' | -47.3 | NC_005083.1 | + | 107130 | 0.94 | 0.184533 |
Target: 5'- aUAAUGUGCUCUAACCAUC-ACCAAUCu -3' miRNA: 3'- -AUUACACGAGAUUGGUAGgUGGUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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