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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22531 | 5' | -45.3 | NC_005083.1 | + | 94955 | 0.66 | 1 |
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Target: 5'- aCGAUGUGAGUUgCGCcucuuGCACGa -3' miRNA: 3'- gGUUACACUUAA-GCGaucuuCGUGUa -5' |
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| 22531 | 5' | -45.3 | NC_005083.1 | + | 178659 | 0.66 | 0.999999 |
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Target: 5'- ------cGGAUUCGCUAGAuacacgaauucugAGCGCAg -3' miRNA: 3'- gguuacaCUUAAGCGAUCU-------------UCGUGUa -5' |
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| 22531 | 5' | -45.3 | NC_005083.1 | + | 114279 | 0.67 | 0.999996 |
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Target: 5'- aCCAAacUGUGAGUUUGUUcaAGAuGGUACAc -3' miRNA: 3'- -GGUU--ACACUUAAGCGA--UCU-UCGUGUa -5' |
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| 22531 | 5' | -45.3 | NC_005083.1 | + | 74324 | 0.67 | 0.999996 |
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Target: 5'- --uGUGUGGAUUauuugcUGCUAGAAGCAa-- -3' miRNA: 3'- gguUACACUUAA------GCGAUCUUCGUgua -5' |
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| 22531 | 5' | -45.3 | NC_005083.1 | + | 147793 | 0.68 | 0.999988 |
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Target: 5'- gCCGA-GUGAAUUUGCUcGAcuGGCGCc- -3' miRNA: 3'- -GGUUaCACUUAAGCGAuCU--UCGUGua -5' |
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| 22531 | 5' | -45.3 | NC_005083.1 | + | 241826 | 0.7 | 0.999868 |
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Target: 5'- ----cGUGAAUUCG-UAGAAGUACGa -3' miRNA: 3'- gguuaCACUUAAGCgAUCUUCGUGUa -5' |
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| 22531 | 5' | -45.3 | NC_005083.1 | + | 107094 | 1.09 | 0.048914 |
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Target: 5'- gCCAAUGUGAAUUCGCUAGAAGCACAUc -3' miRNA: 3'- -GGUUACACUUAAGCGAUCUUCGUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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