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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22532 | 3' | -49.6 | NC_005083.1 | + | 95054 | 0.66 | 0.999301 |
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Target: 5'- cUUCUCGUUGAGCGUaaguucaacccagGCA---AAGGCu -3' miRNA: 3'- -AAGAGCAACUCGCAg------------CGUaacUUCUG- -5' |
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| 22532 | 3' | -49.6 | NC_005083.1 | + | 5299 | 0.66 | 0.999032 |
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Target: 5'- cUUCUCGUuuUGAGCGUCGUcgauuucuuUUGcuGAUg -3' miRNA: 3'- -AAGAGCA--ACUCGCAGCGu--------AACuuCUG- -5' |
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| 22532 | 3' | -49.6 | NC_005083.1 | + | 65452 | 0.67 | 0.997559 |
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Target: 5'- -aCUaGUUGAGCGUCGCGgguUUGAAu-- -3' miRNA: 3'- aaGAgCAACUCGCAGCGU---AACUUcug -5' |
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| 22532 | 3' | -49.6 | NC_005083.1 | + | 213242 | 0.7 | 0.985815 |
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Target: 5'- -cCUUGUUGAGCGaacaauuUCGgGUUGAuacAGACg -3' miRNA: 3'- aaGAGCAACUCGC-------AGCgUAACU---UCUG- -5' |
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| 22532 | 3' | -49.6 | NC_005083.1 | + | 126135 | 1.09 | 0.016939 |
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Target: 5'- gUUCUCGUUGAGCGUCGCAUUGAAGACa -3' miRNA: 3'- -AAGAGCAACUCGCAGCGUAACUUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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