miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22532 3' -49.6 NC_005083.1 + 95054 0.66 0.999301
Target:  5'- cUUCUCGUUGAGCGUaaguucaacccagGCA---AAGGCu -3'
miRNA:   3'- -AAGAGCAACUCGCAg------------CGUaacUUCUG- -5'
22532 3' -49.6 NC_005083.1 + 5299 0.66 0.999032
Target:  5'- cUUCUCGUuuUGAGCGUCGUcgauuucuuUUGcuGAUg -3'
miRNA:   3'- -AAGAGCA--ACUCGCAGCGu--------AACuuCUG- -5'
22532 3' -49.6 NC_005083.1 + 65452 0.67 0.997559
Target:  5'- -aCUaGUUGAGCGUCGCGgguUUGAAu-- -3'
miRNA:   3'- aaGAgCAACUCGCAGCGU---AACUUcug -5'
22532 3' -49.6 NC_005083.1 + 213242 0.7 0.985815
Target:  5'- -cCUUGUUGAGCGaacaauuUCGgGUUGAuacAGACg -3'
miRNA:   3'- aaGAGCAACUCGC-------AGCgUAACU---UCUG- -5'
22532 3' -49.6 NC_005083.1 + 126135 1.09 0.016939
Target:  5'- gUUCUCGUUGAGCGUCGCAUUGAAGACa -3'
miRNA:   3'- -AAGAGCAACUCGCAGCGUAACUUCUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.