miRNA display CGI


Results 1 - 17 of 17 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22532 5' -45.6 NC_005083.1 + 236888 0.66 0.999999
Target:  5'- cGCcuUCUUUGAUAGguugCUUGCAUaCAACa -3'
miRNA:   3'- -CGu-AGAAGUUAUCa---GAGCGUGaGUUG- -5'
22532 5' -45.6 NC_005083.1 + 10557 0.66 0.999999
Target:  5'- aGCAUCUUCGuu--UCUgGCugUgAGCa -3'
miRNA:   3'- -CGUAGAAGUuaucAGAgCGugAgUUG- -5'
22532 5' -45.6 NC_005083.1 + 209444 0.66 0.999999
Target:  5'- uGCAUCgcCAAUcuUCUCGUugUuCAACu -3'
miRNA:   3'- -CGUAGaaGUUAucAGAGCGugA-GUUG- -5'
22532 5' -45.6 NC_005083.1 + 1427 0.66 0.999999
Target:  5'- uGCAaugUCUUUGAUAagaUCGCGCUCAGa -3'
miRNA:   3'- -CGU---AGAAGUUAUcagAGCGUGAGUUg -5'
22532 5' -45.6 NC_005083.1 + 96065 0.66 0.999999
Target:  5'- uGCAUUacguaguugUUCAAUuGUCUCGCAagagUUAACa -3'
miRNA:   3'- -CGUAG---------AAGUUAuCAGAGCGUg---AGUUG- -5'
22532 5' -45.6 NC_005083.1 + 77421 0.67 0.999997
Target:  5'- cCGUUUUCGA-AGUaUCGCACUuCAACu -3'
miRNA:   3'- cGUAGAAGUUaUCAgAGCGUGA-GUUG- -5'
22532 5' -45.6 NC_005083.1 + 223565 0.67 0.999997
Target:  5'- aCGUUUcCGcUAGUUUCGCaaACUCAGCg -3'
miRNA:   3'- cGUAGAaGUuAUCAGAGCG--UGAGUUG- -5'
22532 5' -45.6 NC_005083.1 + 87619 0.67 0.999996
Target:  5'- --uUCUUCAGUucuuugaAGUCUuuaCGCGCUUGACc -3'
miRNA:   3'- cguAGAAGUUA-------UCAGA---GCGUGAGUUG- -5'
22532 5' -45.6 NC_005083.1 + 90505 0.67 0.999994
Target:  5'- gGCAUUUaaaGGUGGUCgUgGCACUCAGa -3'
miRNA:   3'- -CGUAGAag-UUAUCAG-AgCGUGAGUUg -5'
22532 5' -45.6 NC_005083.1 + 110125 0.67 0.999992
Target:  5'- aGCAUUUUCAGcaacaacUGGUCUUGUGCUUu-- -3'
miRNA:   3'- -CGUAGAAGUU-------AUCAGAGCGUGAGuug -5'
22532 5' -45.6 NC_005083.1 + 66676 0.68 0.999988
Target:  5'- uGCAg--UCAAUgaaagcugaAGUCUCauucaaaGCACUCGACg -3'
miRNA:   3'- -CGUagaAGUUA---------UCAGAG-------CGUGAGUUG- -5'
22532 5' -45.6 NC_005083.1 + 193860 0.68 0.999971
Target:  5'- uGUAUCgcuUCGAagcgccUAGUC-CGCugUCAACu -3'
miRNA:   3'- -CGUAGa--AGUU------AUCAGaGCGugAGUUG- -5'
22532 5' -45.6 NC_005083.1 + 73616 0.69 0.99993
Target:  5'- aGUAUCUguucuaagCGGUGG-CUUGgACUCAACa -3'
miRNA:   3'- -CGUAGAa-------GUUAUCaGAGCgUGAGUUG- -5'
22532 5' -45.6 NC_005083.1 + 159744 0.71 0.999363
Target:  5'- uCGUCUUCAucauuAUAGUCUaaaCACUCAAUa -3'
miRNA:   3'- cGUAGAAGU-----UAUCAGAgc-GUGAGUUG- -5'
22532 5' -45.6 NC_005083.1 + 20682 0.73 0.997514
Target:  5'- -gAUCUUUAAUGGUCUuaCGCAgUCGAa -3'
miRNA:   3'- cgUAGAAGUUAUCAGA--GCGUgAGUUg -5'
22532 5' -45.6 NC_005083.1 + 49735 0.74 0.992461
Target:  5'- aGCGUgaaUUCGAUAuGUCUCGCAUUCGc- -3'
miRNA:   3'- -CGUAg--AAGUUAU-CAGAGCGUGAGUug -5'
22532 5' -45.6 NC_005083.1 + 126101 1.15 0.021712
Target:  5'- uGCAUCUUCAAUAGUCUCGCACUCAACa -3'
miRNA:   3'- -CGUAGAAGUUAUCAGAGCGUGAGUUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.