Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22533 | 3' | -43.9 | NC_005083.1 | + | 196568 | 0.66 | 1 |
Target: 5'- gAUAUGGGUUAuGCucCAAGCucuuuGUcGGCa -3' miRNA: 3'- -UAUACCCAAUcUGu-GUUCGu----UAuUCG- -5' |
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22533 | 3' | -43.9 | NC_005083.1 | + | 212902 | 0.66 | 1 |
Target: 5'- -gGUGGGaUGGugACG-GCGAUuGGUa -3' miRNA: 3'- uaUACCCaAUCugUGUuCGUUAuUCG- -5' |
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22533 | 3' | -43.9 | NC_005083.1 | + | 153923 | 0.66 | 1 |
Target: 5'- --uUGGGUaau-UACAGGUggUGAGCc -3' miRNA: 3'- uauACCCAaucuGUGUUCGuuAUUCG- -5' |
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22533 | 3' | -43.9 | NC_005083.1 | + | 182983 | 0.66 | 1 |
Target: 5'- --uUGGuaucaaaUUAGACG-AAGCAAUAAGCa -3' miRNA: 3'- uauACCc------AAUCUGUgUUCGUUAUUCG- -5' |
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22533 | 3' | -43.9 | NC_005083.1 | + | 224866 | 0.66 | 1 |
Target: 5'- --uUGGc-UGGACACcagcaauaucgaAAGCGAUAAGCu -3' miRNA: 3'- uauACCcaAUCUGUG------------UUCGUUAUUCG- -5' |
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22533 | 3' | -43.9 | NC_005083.1 | + | 4531 | 0.67 | 1 |
Target: 5'- ------cUUAGACAUAAGCuGUAGGCg -3' miRNA: 3'- uauacccAAUCUGUGUUCGuUAUUCG- -5' |
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22533 | 3' | -43.9 | NC_005083.1 | + | 242788 | 0.67 | 1 |
Target: 5'- cUAUGGGUU--GCuaaGAGCAcUGAGCu -3' miRNA: 3'- uAUACCCAAucUGug-UUCGUuAUUCG- -5' |
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22533 | 3' | -43.9 | NC_005083.1 | + | 107684 | 0.68 | 0.999999 |
Target: 5'- -cAUGGGUacuGACGCugaAGGCGAagcauuUGAGCa -3' miRNA: 3'- uaUACCCAau-CUGUG---UUCGUU------AUUCG- -5' |
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22533 | 3' | -43.9 | NC_005083.1 | + | 216700 | 0.69 | 0.999997 |
Target: 5'- ---cGGGUUcAGACGCAGuacGUggUAAGUc -3' miRNA: 3'- uauaCCCAA-UCUGUGUU---CGuuAUUCG- -5' |
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22533 | 3' | -43.9 | NC_005083.1 | + | 191686 | 0.7 | 0.999982 |
Target: 5'- cGUAaGGGUgcuGAUACGGGCGAUGguaaauggacuucuGGCa -3' miRNA: 3'- -UAUaCCCAau-CUGUGUUCGUUAU--------------UCG- -5' |
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22533 | 3' | -43.9 | NC_005083.1 | + | 150781 | 0.7 | 0.999973 |
Target: 5'- uUGUGcguuuGGUUGGcACACGugggaauauacgaaaGGCAGUAAGCg -3' miRNA: 3'- uAUAC-----CCAAUC-UGUGU---------------UCGUUAUUCG- -5' |
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22533 | 3' | -43.9 | NC_005083.1 | + | 184181 | 1.11 | 0.047513 |
Target: 5'- uAUAUGGGUUAGACACAAGCAAUAAGCc -3' miRNA: 3'- -UAUACCCAAUCUGUGUUCGUUAUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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