Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22535 | 3' | -53.6 | NC_005083.1 | + | 240936 | 0.66 | 0.990027 |
Target: 5'- cCUGcACCAgCCGaua--GAGCUUGGUg -3' miRNA: 3'- -GACuUGGUgGGCgucaaCUCGAACCG- -5' |
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22535 | 3' | -53.6 | NC_005083.1 | + | 56816 | 0.66 | 0.990027 |
Target: 5'- uUGAACCACUguCAcUUGAGCUUGaGUa -3' miRNA: 3'- gACUUGGUGGgcGUcAACUCGAAC-CG- -5' |
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22535 | 3' | -53.6 | NC_005083.1 | + | 163595 | 0.66 | 0.983495 |
Target: 5'- -cGAGCCgaaguauACCC-CAGUUGuaaaugcGGCUUGGUc -3' miRNA: 3'- gaCUUGG-------UGGGcGUCAAC-------UCGAACCG- -5' |
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22535 | 3' | -53.6 | NC_005083.1 | + | 187981 | 0.67 | 0.979867 |
Target: 5'- cCUGAGCCGaaaagagaaGCAGUUGAcGCaagGGCa -3' miRNA: 3'- -GACUUGGUggg------CGUCAACU-CGaa-CCG- -5' |
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22535 | 3' | -53.6 | NC_005083.1 | + | 20703 | 0.67 | 0.975175 |
Target: 5'- -cGAAUCAUUgGCGaaaaGAGCUUGGCa -3' miRNA: 3'- gaCUUGGUGGgCGUcaa-CUCGAACCG- -5' |
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22535 | 3' | -53.6 | NC_005083.1 | + | 69664 | 0.67 | 0.975175 |
Target: 5'- uUGAuACCAgCCGCGacuaucAGCUUGGCa -3' miRNA: 3'- gACU-UGGUgGGCGUcaac--UCGAACCG- -5' |
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22535 | 3' | -53.6 | NC_005083.1 | + | 89803 | 0.67 | 0.975175 |
Target: 5'- uCUGAGCCGCuuGCAGcacGcGCUUGauauGCa -3' miRNA: 3'- -GACUUGGUGggCGUCaa-CuCGAAC----CG- -5' |
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22535 | 3' | -53.6 | NC_005083.1 | + | 203298 | 0.69 | 0.94841 |
Target: 5'- uCUGccaauAGCCACCUGCAaaaGGGCgUGGCg -3' miRNA: 3'- -GAC-----UUGGUGGGCGUcaaCUCGaACCG- -5' |
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22535 | 3' | -53.6 | NC_005083.1 | + | 141257 | 0.71 | 0.887968 |
Target: 5'- uUGAAUgGCuuGCuGaagaccguaUUGAGCUUGGCg -3' miRNA: 3'- gACUUGgUGggCGuC---------AACUCGAACCG- -5' |
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22535 | 3' | -53.6 | NC_005083.1 | + | 74114 | 0.75 | 0.676813 |
Target: 5'- gCUGAugaaAUCGCUauCGguGUUGAGCUUGGUg -3' miRNA: 3'- -GACU----UGGUGG--GCguCAACUCGAACCG- -5' |
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22535 | 3' | -53.6 | NC_005083.1 | + | 203332 | 1.13 | 0.004183 |
Target: 5'- cCUGAACCACCCGCAGUUGAGCUUGGCg -3' miRNA: 3'- -GACUUGGUGGGCGUCAACUCGAACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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