miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22536 3' -51.5 NC_005083.1 + 166282 0.66 0.995556
Target:  5'- gGAAGAUagcugCAUCGagccgauuUUGCCGCCAGa--- -3'
miRNA:   3'- aCUUCUA-----GUAGC--------AACGGCGGUCagac -5'
22536 3' -51.5 NC_005083.1 + 209714 0.66 0.995556
Target:  5'- cUGAAcuUCAUacucGCCGCCAGUUUGg -3'
miRNA:   3'- -ACUUcuAGUAgcaaCGGCGGUCAGAC- -5'
22536 3' -51.5 NC_005083.1 + 89464 0.67 0.994763
Target:  5'- -uGAGAUCA-CGUUugaagaaGCUGCCGGUCg- -3'
miRNA:   3'- acUUCUAGUaGCAA-------CGGCGGUCAGac -5'
22536 3' -51.5 NC_005083.1 + 30538 0.67 0.994032
Target:  5'- -------aGUCGUUGCUGCUAGUUUGu -3'
miRNA:   3'- acuucuagUAGCAACGGCGGUCAGAC- -5'
22536 3' -51.5 NC_005083.1 + 73807 0.68 0.98835
Target:  5'- gUGAAG-UCGUCG----CGCCAGUCUGu -3'
miRNA:   3'- -ACUUCuAGUAGCaacgGCGGUCAGAC- -5'
22536 3' -51.5 NC_005083.1 + 208635 1.08 0.013459
Target:  5'- cUGAAGAUCAUCGUUGCCGCCAGUCUGc -3'
miRNA:   3'- -ACUUCUAGUAGCAACGGCGGUCAGAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.