Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22537 | 3' | -51.3 | NC_005083.1 | + | 141283 | 0.66 | 0.996589 |
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Target: 5'- -gAGCUuggCGGCGAACGCgCUgaaUGGUUu- -3' miRNA: 3'- agUCGAa--GCCGCUUGCG-GA---ACUAAca -5' |
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| 22537 | 3' | -51.3 | NC_005083.1 | + | 237479 | 0.67 | 0.993769 |
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Target: 5'- -gAGCUUguaGGCGuguUGCUUUGAUUGUu -3' miRNA: 3'- agUCGAAg--CCGCuu-GCGGAACUAACA- -5' |
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| 22537 | 3' | -51.3 | NC_005083.1 | + | 7498 | 0.69 | 0.973417 |
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Target: 5'- aUAGCguucuacaaCGGCGAAUGCCUUGGcgagggUGUg -3' miRNA: 3'- aGUCGaa-------GCCGCUUGCGGAACUa-----ACA- -5' |
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| 22537 | 3' | -51.3 | NC_005083.1 | + | 121178 | 0.69 | 0.973417 |
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Target: 5'- aUUAGUUUCGGUGGugGCUUgcgaAUUGUa -3' miRNA: 3'- -AGUCGAAGCCGCUugCGGAac--UAACA- -5' |
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| 22537 | 3' | -51.3 | NC_005083.1 | + | 51720 | 0.73 | 0.894839 |
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Target: 5'- uUCAGCUUCGGUuuacGAAUGCCUUcGUUa- -3' miRNA: 3'- -AGUCGAAGCCG----CUUGCGGAAcUAAca -5' |
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| 22537 | 3' | -51.3 | NC_005083.1 | + | 222570 | 1.09 | 0.01217 |
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Target: 5'- uUCAGCUUCGGCGAACGCCUUGAUUGUg -3' miRNA: 3'- -AGUCGAAGCCGCUUGCGGAACUAACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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