Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22537 | 5' | -49.4 | NC_005083.1 | + | 103281 | 0.66 | 0.999774 |
Target: 5'- aUGACGGUUggGAaauGCCGCCuuaUACu -3' miRNA: 3'- -ACUGCUAGuuCUac-UGGCGGuucAUG- -5' |
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22537 | 5' | -49.4 | NC_005083.1 | + | 75978 | 0.68 | 0.998504 |
Target: 5'- aGAUGAUCAA---GACCGCgCAcucugGGUACa -3' miRNA: 3'- aCUGCUAGUUcuaCUGGCG-GU-----UCAUG- -5' |
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22537 | 5' | -49.4 | NC_005083.1 | + | 115146 | 0.68 | 0.998504 |
Target: 5'- aUGAaguUGAUCA--AUGAcCCGCCGAGUGa -3' miRNA: 3'- -ACU---GCUAGUucUACU-GGCGGUUCAUg -5' |
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22537 | 5' | -49.4 | NC_005083.1 | + | 133285 | 0.69 | 0.995176 |
Target: 5'- aUGACGAaaUCAucAUGAUCauucuuuuGCCAAGUACa -3' miRNA: 3'- -ACUGCU--AGUucUACUGG--------CGGUUCAUG- -5' |
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22537 | 5' | -49.4 | NC_005083.1 | + | 222265 | 0.7 | 0.992548 |
Target: 5'- uUGGCGGUCAucuUGAUCGUCAAGa-- -3' miRNA: 3'- -ACUGCUAGUucuACUGGCGGUUCaug -5' |
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22537 | 5' | -49.4 | NC_005083.1 | + | 34959 | 0.7 | 0.991454 |
Target: 5'- gUGACGAUCAGGcaGACCagcaGUCAcGUACa -3' miRNA: 3'- -ACUGCUAGUUCuaCUGG----CGGUuCAUG- -5' |
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22537 | 5' | -49.4 | NC_005083.1 | + | 221778 | 0.71 | 0.979861 |
Target: 5'- cGAUGucucCAAGcgacUGACCGCCAGGUAg -3' miRNA: 3'- aCUGCua--GUUCu---ACUGGCGGUUCAUg -5' |
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22537 | 5' | -49.4 | NC_005083.1 | + | 222531 | 1.11 | 0.015399 |
Target: 5'- uUGACGAUCAAGAUGACCGCCAAGUACu -3' miRNA: 3'- -ACUGCUAGUUCUACUGGCGGUUCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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