miRNA: 3'- -GC-UCgCGGAAAGUgCAGauGACGCAG- -5'
miRNA: 3'- -GCuCGCGGA---AAGUGCAGA-UGACgCAG- -5'
miRNA: 3'- gcucgCGGAaaGUGCAGAUGACGCAG- -5'
miRNA: 3'- gcUCGCGgAAAGUGCAGAUGACGCag -5'
miRNA: 3'- -GCUCGCGGAAAGUGCAGAUGACGCAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22538 | 3' | -53.1 | NC_005083.1 | + | 89679 | 0.66 | 0.993572 |
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Target: 5'- aCGcAGgGUCUUUCAaGUCgcUUGCGUCu -3' miRNA: 3'- -GC-UCgCGGAAAGUgCAGauGACGCAG- -5' |
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| 22538 | 3' | -53.1 | NC_005083.1 | + | 201627 | 0.69 | 0.949789 |
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Target: 5'- aCGuGCGCCUgcaUUCAUGUUUgACUG-GUCg -3' miRNA: 3'- -GCuCGCGGA---AAGUGCAGA-UGACgCAG- -5' |
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| 22538 | 3' | -53.1 | NC_005083.1 | + | 167919 | 0.7 | 0.926232 |
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Target: 5'- ----aGCCUgaUGCGUUUGCUGCGUCg -3' miRNA: 3'- gcucgCGGAaaGUGCAGAUGACGCAG- -5' |
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| 22538 | 3' | -53.1 | NC_005083.1 | + | 1608 | 0.72 | 0.876506 |
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Target: 5'- -uAGCGUaUUUCGCGUCUAUUGCGc- -3' miRNA: 3'- gcUCGCGgAAAGUGCAGAUGACGCag -5' |
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| 22538 | 3' | -53.1 | NC_005083.1 | + | 225542 | 1.1 | 0.00718 |
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Target: 5'- aCGAGCGCCUUUCACGUCUACUGCGUCu -3' miRNA: 3'- -GCUCGCGGAAAGUGCAGAUGACGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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