Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22538 | 5' | -55.9 | NC_005083.1 | + | 3912 | 0.66 | 0.951803 |
Target: 5'- uGAACACACCuGGCGGUAacuuugCUGCuGCUUg -3' miRNA: 3'- gUUUGUGUGG-UCGUCGUg-----GACG-CGGA- -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 4289 | 0.66 | 0.951803 |
Target: 5'- aCAAGCAaggaaaAUCA-UAGUACCUGCGCUa -3' miRNA: 3'- -GUUUGUg-----UGGUcGUCGUGGACGCGGa -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 225560 | 0.66 | 0.947676 |
Target: 5'- gUAAGCuuCACCAGCccaacgAGCGCCuuucacgucuacUGCGUCUg -3' miRNA: 3'- -GUUUGu-GUGGUCG------UCGUGG------------ACGCGGA- -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 215241 | 0.66 | 0.947676 |
Target: 5'- cCAGACACuaauUCAGUAGCACUaauacCGCCa -3' miRNA: 3'- -GUUUGUGu---GGUCGUCGUGGac---GCGGa -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 195419 | 0.66 | 0.940599 |
Target: 5'- uCAGAUACAUCaAGCAGUACUagacgaucuggguaGCGCCa -3' miRNA: 3'- -GUUUGUGUGG-UCGUCGUGGa-------------CGCGGa -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 41010 | 0.66 | 0.938737 |
Target: 5'- uCAGGCGCugguuauCCAGCcaguGCGCUgucaaacuuUGCGCCUc -3' miRNA: 3'- -GUUUGUGu------GGUCGu---CGUGG---------ACGCGGA- -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 56422 | 0.66 | 0.938737 |
Target: 5'- gAAACACGCUcagaaccaAGCuacucuGCACCUGaGCCg -3' miRNA: 3'- gUUUGUGUGG--------UCGu-----CGUGGACgCGGa -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 168667 | 0.67 | 0.928874 |
Target: 5'- aCAAGuCACACCuGaacgaucuGCACCUGCGUUg -3' miRNA: 3'- -GUUU-GUGUGGuCgu------CGUGGACGCGGa -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 204202 | 0.67 | 0.917515 |
Target: 5'- ----aGCACCGGCAGCAuagagUCUGCuaccgucGCCUa -3' miRNA: 3'- guuugUGUGGUCGUCGU-----GGACG-------CGGA- -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 38928 | 0.67 | 0.906357 |
Target: 5'- aCAAACACgACCAGCAcGCAU-UGCGUg- -3' miRNA: 3'- -GUUUGUG-UGGUCGU-CGUGgACGCGga -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 41283 | 0.68 | 0.880188 |
Target: 5'- -cAGCGCACUGGCuGGauaACCaGCGCCUg -3' miRNA: 3'- guUUGUGUGGUCG-UCg--UGGaCGCGGA- -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 219220 | 0.69 | 0.842691 |
Target: 5'- cCGAACAUccaAUCGuCAGCACCUGCGUUa -3' miRNA: 3'- -GUUUGUG---UGGUcGUCGUGGACGCGGa -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 44212 | 0.69 | 0.826343 |
Target: 5'- uGAACACGCCGGaAGCACCgGaUGCUg -3' miRNA: 3'- gUUUGUGUGGUCgUCGUGGaC-GCGGa -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 172042 | 0.7 | 0.809307 |
Target: 5'- -cAGCAgCACCAGCagcuacuuuAGCACCUGUuuuuGCCg -3' miRNA: 3'- guUUGU-GUGGUCG---------UCGUGGACG----CGGa -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 162190 | 0.7 | 0.782599 |
Target: 5'- uCAAGCACACCAGCAGauguaACUUuuGCUa -3' miRNA: 3'- -GUUUGUGUGGUCGUCg----UGGAcgCGGa -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 118998 | 0.7 | 0.773422 |
Target: 5'- ---cCAUGCCAGCcGCuacAUCUGCGCCUa -3' miRNA: 3'- guuuGUGUGGUCGuCG---UGGACGCGGA- -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 154692 | 0.71 | 0.745187 |
Target: 5'- ---uUACAUUAGCGGCACC-GCGCUa -3' miRNA: 3'- guuuGUGUGGUCGUCGUGGaCGCGGa -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 190115 | 0.71 | 0.742312 |
Target: 5'- uCAAGCugACCGGCAGCAUUUGauugaaugaaugaaCGCUUg -3' miRNA: 3'- -GUUUGugUGGUCGUCGUGGAC--------------GCGGA- -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 194535 | 0.74 | 0.545845 |
Target: 5'- uUAAACGCACCAGCAGCAa--GUGUCa -3' miRNA: 3'- -GUUUGUGUGGUCGUCGUggaCGCGGa -5' |
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22538 | 5' | -55.9 | NC_005083.1 | + | 225508 | 1.06 | 0.005763 |
Target: 5'- uCAAACACACCAGCAGCACCUGCGCCUu -3' miRNA: 3'- -GUUUGUGUGGUCGUCGUGGACGCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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