Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22539 | 5' | -42 | NC_005083.1 | + | 196836 | 0.7 | 1 |
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Target: 5'- uUUCAGUgaaaguGAGUCgAUUACgaucaaAGACCCg -3' miRNA: 3'- -AAGUUAauu---CUCAGaUAAUG------UCUGGG- -5' |
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| 22539 | 5' | -42 | NC_005083.1 | + | 153139 | 0.66 | 1 |
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Target: 5'- -gCAAcUUcAGAGUCUugAUUACAGAUUCu -3' miRNA: 3'- aaGUU-AAuUCUCAGA--UAAUGUCUGGG- -5' |
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| 22539 | 5' | -42 | NC_005083.1 | + | 231821 | 0.68 | 1 |
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Target: 5'- -cCAAUcGAGGGUCUAUUuCAGAUa- -3' miRNA: 3'- aaGUUAaUUCUCAGAUAAuGUCUGgg -5' |
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| 22539 | 5' | -42 | NC_005083.1 | + | 165901 | 0.7 | 1 |
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Target: 5'- uUUCAGUUAAGAGUaCUucaAUUACAucGACUg -3' miRNA: 3'- -AAGUUAAUUCUCA-GA---UAAUGU--CUGGg -5' |
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| 22539 | 5' | -42 | NC_005083.1 | + | 134397 | 0.7 | 1 |
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Target: 5'- aUCAAUaaGAGAGUCUAUuccaUACAGAUa- -3' miRNA: 3'- aAGUUAa-UUCUCAGAUA----AUGUCUGgg -5' |
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| 22539 | 5' | -42 | NC_005083.1 | + | 225807 | 0.77 | 0.997507 |
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Target: 5'- aUUC-GUUAAGGGUCUGUaAUAGACUCu -3' miRNA: 3'- -AAGuUAAUUCUCAGAUAaUGUCUGGG- -5' |
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| 22539 | 5' | -42 | NC_005083.1 | + | 226086 | 1.12 | 0.0686 |
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Target: 5'- uUUCAAUUAAGAGUCUAUUACAGACCCu -3' miRNA: 3'- -AAGUUAAUUCUCAGAUAAUGUCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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