Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22541 | 3' | -41.2 | NC_005083.1 | + | 3466 | 0.68 | 1 |
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Target: 5'- uUUACUGUUCGuguGACGACU---UCgGa -3' miRNA: 3'- -AAUGACAAGUuu-UUGCUGAuauAGgC- -5' |
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| 22541 | 3' | -41.2 | NC_005083.1 | + | 190570 | 0.66 | 1 |
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Target: 5'- gUACaaaaUCAGGGGCGACUAUcgCCc -3' miRNA: 3'- aAUGaca-AGUUUUUGCUGAUAuaGGc -5' |
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| 22541 | 3' | -41.2 | NC_005083.1 | + | 56858 | 0.66 | 1 |
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Target: 5'- -aACUGUUCcacAAGCuGCUGUGUUCa -3' miRNA: 3'- aaUGACAAGuu-UUUGcUGAUAUAGGc -5' |
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| 22541 | 3' | -41.2 | NC_005083.1 | + | 207591 | 0.66 | 1 |
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Target: 5'- cUACUGUUCu--AACGACaccgguugAUGUCuCGg -3' miRNA: 3'- aAUGACAAGuuuUUGCUGa-------UAUAG-GC- -5' |
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| 22541 | 3' | -41.2 | NC_005083.1 | + | 122913 | 0.69 | 1 |
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Target: 5'- aUACUGUUCAGcAGUGACUAg--CCa -3' miRNA: 3'- aAUGACAAGUUuUUGCUGAUauaGGc -5' |
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| 22541 | 3' | -41.2 | NC_005083.1 | + | 181874 | 0.72 | 1 |
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Target: 5'- aUACUGUaaUCAGAcAUGAUUAUAUUCa -3' miRNA: 3'- aAUGACA--AGUUUuUGCUGAUAUAGGc -5' |
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| 22541 | 3' | -41.2 | NC_005083.1 | + | 145751 | 0.73 | 0.999995 |
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Target: 5'- gUUAUUGUacUCGGAGACGAaucGUGUCCGc -3' miRNA: 3'- -AAUGACA--AGUUUUUGCUga-UAUAGGC- -5' |
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| 22541 | 3' | -41.2 | NC_005083.1 | + | 36608 | 0.81 | 0.98872 |
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Target: 5'- -gGCUGUUCAGAAACGAUU---UCCGu -3' miRNA: 3'- aaUGACAAGUUUUUGCUGAuauAGGC- -5' |
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| 22541 | 3' | -41.2 | NC_005083.1 | + | 36306 | 1.08 | 0.148403 |
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Target: 5'- gUUACUGUUCAAAAACGACUAUAUCCGc -3' miRNA: 3'- -AAUGACAAGUUUUUGCUGAUAUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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