Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22543 | 3' | -44.7 | NC_005083.1 | + | 117365 | 0.66 | 1 |
Target: 5'- -cAGCACacuucuuuacugUUCACGaaaacGGUgaACGUGAACACu -3' miRNA: 3'- cuUCGUG------------AAGUGU-----UCA--UGUACUUGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 147981 | 0.66 | 1 |
Target: 5'- uGAAGUACcaaGCGGGUACGUcGGuaGCGCg -3' miRNA: 3'- -CUUCGUGaagUGUUCAUGUA-CU--UGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 228190 | 0.67 | 0.999999 |
Target: 5'- --cGUACUUUAC-AGUACAagGAACAUc -3' miRNA: 3'- cuuCGUGAAGUGuUCAUGUa-CUUGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 72622 | 0.67 | 0.999999 |
Target: 5'- cGGGCGCUcuUUAUGAGguaaACAUGAGCAa -3' miRNA: 3'- cUUCGUGA--AGUGUUCa---UGUACUUGUg -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 234602 | 0.67 | 0.999999 |
Target: 5'- uGGGUuaaGCUUCGCGgugaauuguuAGUuCAUGAACGCa -3' miRNA: 3'- cUUCG---UGAAGUGU----------UCAuGUACUUGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 172567 | 0.68 | 0.999998 |
Target: 5'- uGAuGUACUUUACGGG-AC-UGAACGCu -3' miRNA: 3'- -CUuCGUGAAGUGUUCaUGuACUUGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 210674 | 0.68 | 0.999995 |
Target: 5'- uGGAGCAaaacUCACAAGUugGUGAc--- -3' miRNA: 3'- -CUUCGUga--AGUGUUCAugUACUugug -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 145317 | 0.68 | 0.999994 |
Target: 5'- -cGGCGCUUCGCuucgauacaugcgcgGAGUGCuucauAUGAAUGCg -3' miRNA: 3'- cuUCGUGAAGUG---------------UUCAUG-----UACUUGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 183206 | 0.7 | 0.99995 |
Target: 5'- aGAAGCACUUCAa----GC-UGAACGCa -3' miRNA: 3'- -CUUCGUGAAGUguucaUGuACUUGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 63651 | 0.7 | 0.99995 |
Target: 5'- aAAGCACUUUGCA-GUGCAauuACACa -3' miRNA: 3'- cUUCGUGAAGUGUuCAUGUacuUGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 64300 | 0.7 | 0.99995 |
Target: 5'- --uGCACUgUACGGGUuCAUGggUACa -3' miRNA: 3'- cuuCGUGAaGUGUUCAuGUACuuGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 187001 | 0.7 | 0.999934 |
Target: 5'- uAAGUGCUUCuCAGGUGCA--AACACu -3' miRNA: 3'- cUUCGUGAAGuGUUCAUGUacUUGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 29290 | 0.71 | 0.999805 |
Target: 5'- uGAAGCAUUUCACA---GCGaaaGAACACg -3' miRNA: 3'- -CUUCGUGAAGUGUucaUGUa--CUUGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 141479 | 0.71 | 0.99975 |
Target: 5'- -cAGCAUgUUCACGAGUAUAUGGuuCGCc -3' miRNA: 3'- cuUCGUG-AAGUGUUCAUGUACUu-GUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 12497 | 0.72 | 0.999215 |
Target: 5'- aAAGCACUUCACcgcuaauauguaAAGUACcgcgcuUGAAUACa -3' miRNA: 3'- cUUCGUGAAGUG------------UUCAUGu-----ACUUGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 116083 | 0.74 | 0.995731 |
Target: 5'- cGAGGCACaagCAUGAGUACAUGGgacgaagugaaagACGCu -3' miRNA: 3'- -CUUCGUGaa-GUGUUCAUGUACU-------------UGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 57851 | 0.75 | 0.989739 |
Target: 5'- uGAAGCugaagCACGAGaGCGUGAGCACc -3' miRNA: 3'- -CUUCGugaa-GUGUUCaUGUACUUGUG- -5' |
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22543 | 3' | -44.7 | NC_005083.1 | + | 51330 | 1.13 | 0.030704 |
Target: 5'- cGAAGCACUUCACAAGUACAUGAACACg -3' miRNA: 3'- -CUUCGUGAAGUGUUCAUGUACUUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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