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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22544 | 5' | -57.2 | NC_005083.1 | + | 119001 | 0.69 | 0.824986 |
Target: 5'- uUGCcaUGCCAGCCGCUacauCUGCGCCu- -3' miRNA: 3'- cAUGaaGCGGUUGGUGG----GACGCGGca -5' |
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22544 | 5' | -57.2 | NC_005083.1 | + | 87770 | 0.69 | 0.80823 |
Target: 5'- -cACUU-GCCAuaccuacaaaGCCACCCacugcUGCGCCGa -3' miRNA: 3'- caUGAAgCGGU----------UGGUGGG-----ACGCGGCa -5' |
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22544 | 5' | -57.2 | NC_005083.1 | + | 83892 | 1.07 | 0.004511 |
Target: 5'- gGUACUUCGCCAACCACCCUGCGCCGUg -3' miRNA: 3'- -CAUGAAGCGGUUGGUGGGACGCGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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