Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22545 | 3' | -46.1 | NC_005083.1 | + | 76291 | 0.66 | 0.999996 |
Target: 5'- -gUCgUCGCGaUACACAGccGCGAUauuuuuguagugagUGUGUa -3' miRNA: 3'- uaAG-AGCGC-AUGUGUU--UGCUA--------------ACACA- -5' |
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22545 | 3' | -46.1 | NC_005083.1 | + | 218181 | 0.67 | 0.999995 |
Target: 5'- ---aUCG-GUGCuuCAAACGGUUGUGUu -3' miRNA: 3'- uaagAGCgCAUGu-GUUUGCUAACACA- -5' |
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22545 | 3' | -46.1 | NC_005083.1 | + | 47433 | 0.71 | 0.998883 |
Target: 5'- -aUCUUGCGUuaaGCAAAUGAUUGUu- -3' miRNA: 3'- uaAGAGCGCAug-UGUUUGCUAACAca -5' |
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22545 | 3' | -46.1 | NC_005083.1 | + | 91286 | 1.06 | 0.060116 |
Target: 5'- cAUUCUCGCGUACACAAACGAUUGUGUu -3' miRNA: 3'- -UAAGAGCGCAUGUGUUUGCUAACACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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