Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22545 | 5' | -48.3 | NC_005083.1 | + | 173638 | 0.66 | 0.999924 |
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Target: 5'- uCAACGGCgguaGUCAgcUUCGUguaGAACGCGg-- -3' miRNA: 3'- -GUUGCCG----UAGU--AAGCA---CUUGUGCaca -5' |
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| 22545 | 5' | -48.3 | NC_005083.1 | + | 146044 | 0.66 | 0.999901 |
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Target: 5'- -cAUGG-AUCAUUCGUucccuGCACGUGUa -3' miRNA: 3'- guUGCCgUAGUAAGCAcu---UGUGCACA- -5' |
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| 22545 | 5' | -48.3 | NC_005083.1 | + | 232317 | 0.67 | 0.99979 |
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Target: 5'- aAGCGcGCcugcuuucuuGUCAUcgUCGUGAACACGUu- -3' miRNA: 3'- gUUGC-CG----------UAGUA--AGCACUUGUGCAca -5' |
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| 22545 | 5' | -48.3 | NC_005083.1 | + | 180132 | 0.67 | 0.999734 |
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Target: 5'- aCAACaGCGUCGUUCG-GAAuCACGa-- -3' miRNA: 3'- -GUUGcCGUAGUAAGCaCUU-GUGCaca -5' |
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| 22545 | 5' | -48.3 | NC_005083.1 | + | 75802 | 0.69 | 0.998824 |
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Target: 5'- aGGCGGCAUCGUUcuaggaCGUGcAACACaagGUGa -3' miRNA: 3'- gUUGCCGUAGUAA------GCAC-UUGUG---CACa -5' |
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| 22545 | 5' | -48.3 | NC_005083.1 | + | 235810 | 0.7 | 0.99731 |
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Target: 5'- uGACGGCAUCAUcaaaaaaaugaccagUUGUGAAUACa--- -3' miRNA: 3'- gUUGCCGUAGUA---------------AGCACUUGUGcaca -5' |
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| 22545 | 5' | -48.3 | NC_005083.1 | + | 21240 | 0.73 | 0.972869 |
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Target: 5'- uCAGCGGCAUCAUacCGUGAACAa---- -3' miRNA: 3'- -GUUGCCGUAGUAa-GCACUUGUgcaca -5' |
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| 22545 | 5' | -48.3 | NC_005083.1 | + | 91321 | 1.08 | 0.028193 |
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Target: 5'- uCAACGGCAUCAUUCGUGAACACGUGUa -3' miRNA: 3'- -GUUGCCGUAGUAAGCACUUGUGCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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