Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22546 | 3' | -50.3 | NC_005083.1 | + | 114182 | 0.66 | 0.999534 |
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Target: 5'- cGCUCGGGCugUagaacUCGUGAGAAUc -3' miRNA: 3'- -CGAGUUCGugAagccaAGCGCUCUUG- -5' |
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| 22546 | 3' | -50.3 | NC_005083.1 | + | 81955 | 0.66 | 0.999534 |
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Target: 5'- cGCUuCAAGUGCUUCa--UCGUGAGAuACg -3' miRNA: 3'- -CGA-GUUCGUGAAGccaAGCGCUCU-UG- -5' |
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| 22546 | 3' | -50.3 | NC_005083.1 | + | 194788 | 0.66 | 0.999292 |
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Target: 5'- -gUCAAGCAUUUCGGUagGUacGAAUa -3' miRNA: 3'- cgAGUUCGUGAAGCCAagCGcuCUUG- -5' |
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| 22546 | 3' | -50.3 | NC_005083.1 | + | 4572 | 0.66 | 0.998948 |
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Target: 5'- -gUCGAGCACUUCuGGUUaCGCGc---- -3' miRNA: 3'- cgAGUUCGUGAAG-CCAA-GCGCucuug -5' |
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| 22546 | 3' | -50.3 | NC_005083.1 | + | 119815 | 0.66 | 0.998728 |
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Target: 5'- -gUCAAuaACUUCGGUacUgGCGaAGAACa -3' miRNA: 3'- cgAGUUcgUGAAGCCA--AgCGC-UCUUG- -5' |
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| 22546 | 3' | -50.3 | NC_005083.1 | + | 113525 | 0.67 | 0.998471 |
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Target: 5'- cGCUUGAGCACUUgaGGcugaaCGCGAGucauGACa -3' miRNA: 3'- -CGAGUUCGUGAAg-CCaa---GCGCUC----UUG- -5' |
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| 22546 | 3' | -50.3 | NC_005083.1 | + | 138287 | 0.67 | 0.998471 |
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Target: 5'- uGUUCAaaaAGCACUUCGGUUC-UGAa--- -3' miRNA: 3'- -CGAGU---UCGUGAAGCCAAGcGCUcuug -5' |
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| 22546 | 3' | -50.3 | NC_005083.1 | + | 14366 | 0.68 | 0.995822 |
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Target: 5'- gGUUCAGGCucgcguuCUUUGcGUUCGCGuGAGu -3' miRNA: 3'- -CGAGUUCGu------GAAGC-CAAGCGCuCUUg -5' |
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| 22546 | 3' | -50.3 | NC_005083.1 | + | 39763 | 0.73 | 0.917559 |
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Target: 5'- gGUUCAGGUGCUUCGGUa-GUGuGGACa -3' miRNA: 3'- -CGAGUUCGUGAAGCCAagCGCuCUUG- -5' |
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| 22546 | 3' | -50.3 | NC_005083.1 | + | 113884 | 1.13 | 0.009047 |
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Target: 5'- uGCUCAAGCACUUCGGUUCGCGAGAACg -3' miRNA: 3'- -CGAGUUCGUGAAGCCAAGCGCUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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