Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22547 | 5' | -48.9 | NC_005083.1 | + | 148893 | 0.66 | 0.999805 |
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Target: 5'- cUCAGUgcaGGGUCGUGCGCcuaauaucgcguucuUGCCuAUCu -3' miRNA: 3'- -AGUCAac-UCUAGUACGCG---------------AUGG-UAGu -5' |
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| 22547 | 5' | -48.9 | NC_005083.1 | + | 44666 | 0.66 | 0.999727 |
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Target: 5'- gUCAgcGUUGcGAUCuGUGCGuCUGCCAuaUCAa -3' miRNA: 3'- -AGU--CAACuCUAG-UACGC-GAUGGU--AGU- -5' |
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| 22547 | 5' | -48.9 | NC_005083.1 | + | 89456 | 0.68 | 0.99879 |
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Target: 5'- uUCGGUcuUGAGAUCAcguuUGaagaaGCUGCCgGUCGu -3' miRNA: 3'- -AGUCA--ACUCUAGU----ACg----CGAUGG-UAGU- -5' |
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| 22547 | 5' | -48.9 | NC_005083.1 | + | 142645 | 0.68 | 0.99851 |
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Target: 5'- --cGUUGuaaucaaAGAUgAUGUGCUACUAUCAc -3' miRNA: 3'- aguCAAC-------UCUAgUACGCGAUGGUAGU- -5' |
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| 22547 | 5' | -48.9 | NC_005083.1 | + | 164922 | 0.73 | 0.952558 |
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Target: 5'- cCAGUUGAGAUCaAUGUcccauugacugugaaGCUuCCAUCAg -3' miRNA: 3'- aGUCAACUCUAG-UACG---------------CGAuGGUAGU- -5' |
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| 22547 | 5' | -48.9 | NC_005083.1 | + | 119506 | 1.1 | 0.017776 |
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Target: 5'- cUCAGUUGAGAUCAUGCGCUACCAUCAu -3' miRNA: 3'- -AGUCAACUCUAGUACGCGAUGGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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