Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22548 | 3' | -45.4 | NC_005083.1 | + | 94931 | 0.66 | 1 |
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Target: 5'- cGAGAAcgaacauuuGACuGCCAaACGucGUCUUUCGc -3' miRNA: 3'- aCUCUU---------UUG-CGGUaUGCu-CAGAAAGU- -5' |
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| 22548 | 3' | -45.4 | NC_005083.1 | + | 225209 | 0.66 | 1 |
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Target: 5'- cUGAGAAGuUGCCAUACcagcGUCgaUUUCAa -3' miRNA: 3'- -ACUCUUUuGCGGUAUGcu--CAG--AAAGU- -5' |
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| 22548 | 3' | -45.4 | NC_005083.1 | + | 201606 | 0.67 | 0.999998 |
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Target: 5'- aUGGGGAauGACGCCGUACGAacgugcGcCUgcaUUCAu -3' miRNA: 3'- -ACUCUU--UUGCGGUAUGCU------CaGA---AAGU- -5' |
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| 22548 | 3' | -45.4 | NC_005083.1 | + | 203052 | 0.68 | 0.999995 |
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Target: 5'- aGAGAAuucCGCCAagcucaacUGCGGGUggUUCAg -3' miRNA: 3'- aCUCUUuu-GCGGU--------AUGCUCAgaAAGU- -5' |
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| 22548 | 3' | -45.4 | NC_005083.1 | + | 224975 | 0.68 | 0.99998 |
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Target: 5'- aGAGAacgAAGCGUCAgcuugACGAGUCg---- -3' miRNA: 3'- aCUCU---UUUGCGGUa----UGCUCAGaaagu -5' |
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| 22548 | 3' | -45.4 | NC_005083.1 | + | 60075 | 0.68 | 0.99998 |
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Target: 5'- uUGAGAAAauggcagcauuACGCCAUGCacGUCUUcUCGg -3' miRNA: 3'- -ACUCUUU-----------UGCGGUAUGcuCAGAA-AGU- -5' |
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| 22548 | 3' | -45.4 | NC_005083.1 | + | 116384 | 0.69 | 0.999951 |
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Target: 5'- --uGAAGACGCCGUuuacaGcGUCUUUCAc -3' miRNA: 3'- acuCUUUUGCGGUAug---CuCAGAAAGU- -5' |
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| 22548 | 3' | -45.4 | NC_005083.1 | + | 237551 | 0.7 | 0.999855 |
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Target: 5'- -cGGAAAACGCUGUucuUGAGUCUUUa- -3' miRNA: 3'- acUCUUUUGCGGUAu--GCUCAGAAAgu -5' |
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| 22548 | 3' | -45.4 | NC_005083.1 | + | 83293 | 0.71 | 0.999616 |
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Target: 5'- uUGAGAAAACGCCA---GAGUgUggUCAu -3' miRNA: 3'- -ACUCUUUUGCGGUaugCUCAgAa-AGU- -5' |
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| 22548 | 3' | -45.4 | NC_005083.1 | + | 126244 | 1.11 | 0.038128 |
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Target: 5'- uUGAGAAAACGCCAUACGAGUCUUUCAa -3' miRNA: 3'- -ACUCUUUUGCGGUAUGCUCAGAAAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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