Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22551 | 3' | -51.9 | NC_005091.1 | + | 110 | 0.69 | 0.701328 |
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Target: 5'- --cGACGagUGCUUCAUGGAGCACgUCu- -3' miRNA: 3'- cauCUGC--ACGAGGUACUUCGUG-AGca -5' |
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| 22551 | 3' | -51.9 | NC_005091.1 | + | 57504 | 0.69 | 0.701328 |
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Target: 5'- --cGACGagUGCUUCAUGGAGCACgUCu- -3' miRNA: 3'- cauCUGC--ACGAGGUACUUCGUG-AGca -5' |
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| 22551 | 3' | -51.9 | NC_005091.1 | + | 40749 | 0.69 | 0.701328 |
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Target: 5'- ---aACGUGUUCCGUGAagGGCGCUgcgCGUa -3' miRNA: 3'- caucUGCACGAGGUACU--UCGUGA---GCA- -5' |
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| 22551 | 3' | -51.9 | NC_005091.1 | + | 49295 | 0.69 | 0.686809 |
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Target: 5'- -cGGGCGUGCgCCGUacgucgaacgcgucGAuAGCGCUCGUg -3' miRNA: 3'- caUCUGCACGaGGUA--------------CU-UCGUGAGCA- -5' |
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| 22551 | 3' | -51.9 | NC_005091.1 | + | 196 | 1.07 | 0.002698 |
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Target: 5'- cGUAGACGUGCUCCAUGAAGCACUCGUc -3' miRNA: 3'- -CAUCUGCACGAGGUACUUCGUGAGCA- -5' |
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| 22551 | 3' | -51.9 | NC_005091.1 | + | 57589 | 1.07 | 0.002698 |
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Target: 5'- cGUAGACGUGCUCCAUGAAGCACUCGUc -3' miRNA: 3'- -CAUCUGCACGAGGUACUUCGUGAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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