Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22551 | 5' | -60.8 | NC_005091.1 | + | 50818 | 0.66 | 0.438962 |
Target: 5'- aAGCGu-GCGGCCGUcGuGCGCGCGgCUa -3' miRNA: 3'- cUUGCucCGUCGGCA-C-CGCGUGCgGA- -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 24794 | 0.66 | 0.438962 |
Target: 5'- --cCGAGGaauuccGCCGUGcuGCGCAgGCCg -3' miRNA: 3'- cuuGCUCCgu----CGGCAC--CGCGUgCGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 52534 | 0.66 | 0.429614 |
Target: 5'- -cAUGGGGUcGCCGcccGGCGCGC-CCUg -3' miRNA: 3'- cuUGCUCCGuCGGCa--CCGCGUGcGGA- -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 29603 | 0.66 | 0.428686 |
Target: 5'- aGGCGAcGCgcaacgaccucgaAGCUGUgaucGGCGCGCGCCa -3' miRNA: 3'- cUUGCUcCG-------------UCGGCA----CCGCGUGCGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 54926 | 0.66 | 0.420384 |
Target: 5'- -uGCGGGGauuuugGGCUGaUGGCGC-CGCCc -3' miRNA: 3'- cuUGCUCCg-----UCGGC-ACCGCGuGCGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 45800 | 0.66 | 0.420384 |
Target: 5'- cGGAaGAGcaGUAGCCGgcgcGGCGCGCgGCCg -3' miRNA: 3'- -CUUgCUC--CGUCGGCa---CCGCGUG-CGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 52110 | 0.66 | 0.411275 |
Target: 5'- uGAAa-AGGCGGCCGUcgccGGCGCgggGCGCg- -3' miRNA: 3'- -CUUgcUCCGUCGGCA----CCGCG---UGCGga -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 48614 | 0.66 | 0.41037 |
Target: 5'- --uCGGGGCGGCCGUcggggucccauccGGCGagGCGCg- -3' miRNA: 3'- cuuGCUCCGUCGGCA-------------CCGCg-UGCGga -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 36345 | 0.67 | 0.36678 |
Target: 5'- uGGCGucguGGCGGCguucaucuCGUGGCGCAaacucguCGCCa -3' miRNA: 3'- cUUGCu---CCGUCG--------GCACCGCGU-------GCGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 33599 | 0.68 | 0.319588 |
Target: 5'- aAACGcAGGCAGCCGUGaUGCggaucgAUGCCg -3' miRNA: 3'- cUUGC-UCCGUCGGCACcGCG------UGCGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 9553 | 0.68 | 0.319588 |
Target: 5'- uGACGAGGCuGCCGUGuCGgCA-GCCa -3' miRNA: 3'- cUUGCUCCGuCGGCACcGC-GUgCGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 11403 | 0.69 | 0.290271 |
Target: 5'- cGAACGcGGCAGCCGcucaacaaGGCGUugaaGCCg -3' miRNA: 3'- -CUUGCuCCGUCGGCa-------CCGCGug--CGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 26259 | 0.69 | 0.269696 |
Target: 5'- cGAGCGAGGCgagcaucaaaAGCCuUGGCGUuCGCa- -3' miRNA: 3'- -CUUGCUCCG----------UCGGcACCGCGuGCGga -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 31762 | 0.69 | 0.263103 |
Target: 5'- --cCGAGGCGGCCG-GGUuccacggcacgaGCAgGCCg -3' miRNA: 3'- cuuGCUCCGUCGGCaCCG------------CGUgCGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 2428 | 0.71 | 0.215003 |
Target: 5'- uGAACGAGauucGUGGCU-UGGCGCACGCUg -3' miRNA: 3'- -CUUGCUC----CGUCGGcACCGCGUGCGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 16234 | 0.71 | 0.204218 |
Target: 5'- gGGACGAGGCcGCCGgcauGCGaUGCGCCg -3' miRNA: 3'- -CUUGCUCCGuCGGCac--CGC-GUGCGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 23382 | 0.71 | 0.199004 |
Target: 5'- --cCGGGGCAGCCGacgcaGCGC-CGCCg -3' miRNA: 3'- cuuGCUCCGUCGGCac---CGCGuGCGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 17916 | 0.71 | 0.199004 |
Target: 5'- gGAACGuuuGUcGCCGUGGCGCGCaaGCCg -3' miRNA: 3'- -CUUGCuc-CGuCGGCACCGCGUG--CGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 49278 | 0.72 | 0.179292 |
Target: 5'- -cACGAGGCGcaguucGUCG-GGCGUGCGCCg -3' miRNA: 3'- cuUGCUCCGU------CGGCaCCGCGUGCGGa -5' |
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22551 | 5' | -60.8 | NC_005091.1 | + | 55837 | 0.72 | 0.179292 |
Target: 5'- aGAGCGGcGCAGCCGgucagcaacUGGCGCACgagcugcacgGCCUg -3' miRNA: 3'- -CUUGCUcCGUCGGC---------ACCGCGUG----------CGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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