Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22552 | 3' | -45.4 | NC_005091.1 | + | 43975 | 0.66 | 0.997887 |
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Target: 5'- --aGUUCGGccgUGGuguucAGGUCUUCGCGGc -3' miRNA: 3'- ccgUAAGUCa--ACUu----UCCAGAAGUGUC- -5' |
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| 22552 | 3' | -45.4 | NC_005091.1 | + | 27959 | 0.67 | 0.99628 |
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Target: 5'- cGGCAacUUcCAGUUGAAGGG-CgaCGCAu -3' miRNA: 3'- -CCGU--AA-GUCAACUUUCCaGaaGUGUc -5' |
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| 22552 | 3' | -45.4 | NC_005091.1 | + | 3389 | 0.67 | 0.995561 |
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Target: 5'- uGCAaUCAGUUGAAguGGGUCggCgaccuguucaaGCAGg -3' miRNA: 3'- cCGUaAGUCAACUU--UCCAGaaG-----------UGUC- -5' |
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| 22552 | 3' | -45.4 | NC_005091.1 | + | 18955 | 0.67 | 0.993784 |
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Target: 5'- cGGCGUUCc---GggGcGUCUUCACGGu -3' miRNA: 3'- -CCGUAAGucaaCuuUcCAGAAGUGUC- -5' |
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| 22552 | 3' | -45.4 | NC_005091.1 | + | 18145 | 0.7 | 0.97084 |
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Target: 5'- cGGCAagaUUCAGgcgggucgcuacgagGAAGGGUCUUUcCAGa -3' miRNA: 3'- -CCGU---AAGUCaa-------------CUUUCCAGAAGuGUC- -5' |
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| 22552 | 3' | -45.4 | NC_005091.1 | + | 860 | 1.13 | 0.006381 |
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Target: 5'- cGGCAUUCAGUUGAAAGGUCUUCACAGu -3' miRNA: 3'- -CCGUAAGUCAACUUUCCAGAAGUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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