Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22553 | 3' | -62 | NC_005091.1 | + | 18357 | 0.66 | 0.41782 |
Target: 5'- cGUgGGUGCGaUGUauagCGUCacaacggcguACCCGGCa -3' miRNA: 3'- uCAgCCACGCgACGg---GCAG----------UGGGCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 19042 | 0.66 | 0.41514 |
Target: 5'- uAGUCGagaCGCUGCCCGcccacgcaguaagaUCGCCCacGGUg -3' miRNA: 3'- -UCAGCcacGCGACGGGC--------------AGUGGG--CCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 4218 | 0.66 | 0.400156 |
Target: 5'- --cCGGcgGCGC-GCCCGUCACC-GuGCc -3' miRNA: 3'- ucaGCCa-CGCGaCGGGCAGUGGgC-CG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 30820 | 0.66 | 0.374571 |
Target: 5'- aAGUCGGacGUGCUGCCCuuguagaacagGUUcagcagcucgACCaCGGCg -3' miRNA: 3'- -UCAGCCa-CGCGACGGG-----------CAG----------UGG-GCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 26325 | 0.66 | 0.374571 |
Target: 5'- --cUGGcgGC-CUGCUCG-CGCCCGGCc -3' miRNA: 3'- ucaGCCa-CGcGACGGGCaGUGGGCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 4302 | 0.68 | 0.304694 |
Target: 5'- uGGUCGuGUucGCGCcGgCCGUCGucuuccuguuuCCCGGCu -3' miRNA: 3'- -UCAGC-CA--CGCGaCgGGCAGU-----------GGGCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 32019 | 0.68 | 0.297578 |
Target: 5'- aGGUCcuUGCG--GCCCGUCGCCUuGGCg -3' miRNA: 3'- -UCAGccACGCgaCGGGCAGUGGG-CCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 18140 | 0.69 | 0.277007 |
Target: 5'- cGUCGGUcgGCGCgaGCuUCGcgGCCCGGCg -3' miRNA: 3'- uCAGCCA--CGCGa-CG-GGCagUGGGCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 17331 | 0.69 | 0.270406 |
Target: 5'- --cCGcGUaGCGCUGgCCGUUcagcuucagauuGCCCGGCg -3' miRNA: 3'- ucaGC-CA-CGCGACgGGCAG------------UGGGCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 23466 | 0.69 | 0.257586 |
Target: 5'- -uUCGGcgGCGCUGCgUCGgcugC-CCCGGCu -3' miRNA: 3'- ucAGCCa-CGCGACG-GGCa---GuGGGCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 21515 | 0.69 | 0.257586 |
Target: 5'- ---aGcGUGCuCUGCCgCGUCGCCuCGGCu -3' miRNA: 3'- ucagC-CACGcGACGG-GCAGUGG-GCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 37496 | 0.69 | 0.251365 |
Target: 5'- --aUGGcGCGCUGCuuGUCGCUcaguuCGGCu -3' miRNA: 3'- ucaGCCaCGCGACGggCAGUGG-----GCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 14628 | 0.7 | 0.233441 |
Target: 5'- uGGUCGGcGUccaGUUGCaagcagCCGUCGcCCCGGCa -3' miRNA: 3'- -UCAGCCaCG---CGACG------GGCAGU-GGGCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 49683 | 0.7 | 0.216605 |
Target: 5'- -aUCGGUGCGUUGaUCGU--CCCGGCg -3' miRNA: 3'- ucAGCCACGCGACgGGCAguGGGCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 31859 | 0.7 | 0.216605 |
Target: 5'- cGUCGGcgGC-CUGCUCGUgccguggaACCCGGCc -3' miRNA: 3'- uCAGCCa-CGcGACGGGCAg-------UGGGCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 50299 | 0.71 | 0.181336 |
Target: 5'- cGUCGGcuCGCgGCCgaUCACCCGGCa -3' miRNA: 3'- uCAGCCacGCGaCGGgcAGUGGGCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 25228 | 0.74 | 0.125432 |
Target: 5'- cGGUCGGUGCguccggggcggccGCUGCaCCGgggucgaugCAgCCGGCg -3' miRNA: 3'- -UCAGCCACG-------------CGACG-GGCa--------GUgGGCCG- -5' |
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22553 | 3' | -62 | NC_005091.1 | + | 1406 | 1.11 | 0.000189 |
Target: 5'- uAGUCGGUGCGCUGCCCGUCACCCGGCg -3' miRNA: 3'- -UCAGCCACGCGACGGGCAGUGGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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