Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22554 | 5' | -54.2 | NC_005091.1 | + | 55562 | 0.66 | 0.78659 |
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Target: 5'- gUCCCCGGUCUcggUgAUGAaGACGUg -3' miRNA: 3'- gAGGGGCCAGAagaAgUGCUgCUGUA- -5' |
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| 22554 | 5' | -54.2 | NC_005091.1 | + | 43639 | 0.67 | 0.735314 |
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Target: 5'- cCUgUCCGGcgCUUC-UCGCGAUGGCGc -3' miRNA: 3'- -GAgGGGCCa-GAAGaAGUGCUGCUGUa -5' |
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| 22554 | 5' | -54.2 | NC_005091.1 | + | 55890 | 0.67 | 0.724699 |
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Target: 5'- -aUCCCGGUCg----CACGAUGGCGc -3' miRNA: 3'- gaGGGGCCAGaagaaGUGCUGCUGUa -5' |
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| 22554 | 5' | -54.2 | NC_005091.1 | + | 18844 | 0.67 | 0.713991 |
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Target: 5'- --gCCCGGUCUUCUaUCcggagaagGCGACGAUc- -3' miRNA: 3'- gagGGGCCAGAAGA-AG--------UGCUGCUGua -5' |
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| 22554 | 5' | -54.2 | NC_005091.1 | + | 29718 | 0.69 | 0.593277 |
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Target: 5'- aCUCCCCGGagUccCUUCAgCGACGAUu- -3' miRNA: 3'- -GAGGGGCCagAa-GAAGU-GCUGCUGua -5' |
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| 22554 | 5' | -54.2 | NC_005091.1 | + | 7521 | 0.7 | 0.560504 |
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Target: 5'- aUCgCCgGGUCUUCUUCA--GCGGCAg -3' miRNA: 3'- gAG-GGgCCAGAAGAAGUgcUGCUGUa -5' |
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| 22554 | 5' | -54.2 | NC_005091.1 | + | 470 | 0.74 | 0.329615 |
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Target: 5'- uCUCCCCGccgCUUggccgcCUUCACGACGGCGUc -3' miRNA: 3'- -GAGGGGCca-GAA------GAAGUGCUGCUGUA- -5' |
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| 22554 | 5' | -54.2 | NC_005091.1 | + | 57864 | 0.74 | 0.329615 |
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Target: 5'- uCUCCCCGccgCUUggccgcCUUCACGACGGCGUc -3' miRNA: 3'- -GAGGGGCca-GAA------GAAGUGCUGCUGUA- -5' |
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| 22554 | 5' | -54.2 | NC_005091.1 | + | 1918 | 1.06 | 0.002352 |
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Target: 5'- aCUCCCCGGUCUUCUUCACGACGACAUc -3' miRNA: 3'- -GAGGGGCCAGAAGAAGUGCUGCUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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