miRNA: 3'- gGAGCG-GUCAaGGCGaAGCCGGCUGc -5'
miRNA: 3'- -GGaGCGGUcaa--GGCGAag-CCGGCUGC- -5'
miRNA: 3'- -GGAGCG-GuCAAGGCGaAGCCGGCUGc -5'
miRNA: 3'- ggAGCGGUCaaGGCGa----AGCCGGCUGc -5'
miRNA: 3'- -GGAGCGGUCAAGGCGAAGCCGGCUGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22555 | 3' | -57.8 | NC_005091.1 | + | 48786 | 0.72 | 0.290325 |
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Target: 5'- gCUCGUgCAGUgCCGCaUUGGCCGGCc -3' miRNA: 3'- gGAGCG-GUCAaGGCGaAGCCGGCUGc -5' |
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| 22555 | 3' | -57.8 | NC_005091.1 | + | 11756 | 0.73 | 0.27652 |
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Target: 5'- gCCgCGCCAucgagCCGCUgaaGGCCGGCGa -3' miRNA: 3'- -GGaGCGGUcaa--GGCGAag-CCGGCUGC- -5' |
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| 22555 | 3' | -57.8 | NC_005091.1 | + | 42103 | 0.73 | 0.263244 |
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Target: 5'- uCUUCGCgC-GUUCCGUcUCGGCCGAUc -3' miRNA: 3'- -GGAGCG-GuCAAGGCGaAGCCGGCUGc -5' |
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| 22555 | 3' | -57.8 | NC_005091.1 | + | 18229 | 0.75 | 0.19931 |
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Target: 5'- -aUCGCCGGg-CCGCgaagcUCGGCCGACc -3' miRNA: 3'- ggAGCGGUCaaGGCGa----AGCCGGCUGc -5' |
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| 22555 | 3' | -57.8 | NC_005091.1 | + | 2227 | 1.09 | 0.000726 |
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Target: 5'- uCCUCGCCAGUUCCGCUUCGGCCGACGc -3' miRNA: 3'- -GGAGCGGUCAAGGCGAAGCCGGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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