Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22555 | 5' | -52.9 | NC_005091.1 | + | 30510 | 0.67 | 0.790085 |
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Target: 5'- gACCAUCAGcgGCGCGcuCUgCGUcaUGAGc -3' miRNA: 3'- -UGGUAGUCaaCGCGCcuGA-GCA--ACUC- -5' |
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| 22555 | 5' | -52.9 | NC_005091.1 | + | 26448 | 0.68 | 0.759512 |
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Target: 5'- cCCGUCAGgaccgcGCGCGGGC-CGccGGGg -3' miRNA: 3'- uGGUAGUCaa----CGCGCCUGaGCaaCUC- -5' |
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| 22555 | 5' | -52.9 | NC_005091.1 | + | 46553 | 0.7 | 0.628337 |
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Target: 5'- gACCAUCAGcucGCGCGacGGCUUGUcGAGc -3' miRNA: 3'- -UGGUAGUCaa-CGCGC--CUGAGCAaCUC- -5' |
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| 22555 | 5' | -52.9 | NC_005091.1 | + | 41844 | 0.71 | 0.561703 |
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Target: 5'- aGCCAUCAGgauuucaGUGGACUCGUgcgccGAGu -3' miRNA: 3'- -UGGUAGUCaacg---CGCCUGAGCAa----CUC- -5' |
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| 22555 | 5' | -52.9 | NC_005091.1 | + | 47564 | 0.75 | 0.344799 |
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Target: 5'- uCCG-CAG-UGCcccgGCGGACUCGUUGAGa -3' miRNA: 3'- uGGUaGUCaACG----CGCCUGAGCAACUC- -5' |
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| 22555 | 5' | -52.9 | NC_005091.1 | + | 2190 | 1.09 | 0.001783 |
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Target: 5'- cACCAUCAGUUGCGCGGACUCGUUGAGa -3' miRNA: 3'- -UGGUAGUCAACGCGCCUGAGCAACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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