miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22555 5' -52.9 NC_005091.1 + 26448 0.68 0.759512
Target:  5'- cCCGUCAGgaccgcGCGCGGGC-CGccGGGg -3'
miRNA:   3'- uGGUAGUCaa----CGCGCCUGaGCaaCUC- -5'
22555 5' -52.9 NC_005091.1 + 46553 0.7 0.628337
Target:  5'- gACCAUCAGcucGCGCGacGGCUUGUcGAGc -3'
miRNA:   3'- -UGGUAGUCaa-CGCGC--CUGAGCAaCUC- -5'
22555 5' -52.9 NC_005091.1 + 47564 0.75 0.344799
Target:  5'- uCCG-CAG-UGCcccgGCGGACUCGUUGAGa -3'
miRNA:   3'- uGGUaGUCaACG----CGCCUGAGCAACUC- -5'
22555 5' -52.9 NC_005091.1 + 2190 1.09 0.001783
Target:  5'- cACCAUCAGUUGCGCGGACUCGUUGAGa -3'
miRNA:   3'- -UGGUAGUCAACGCGCCUGAGCAACUC- -5'
22555 5' -52.9 NC_005091.1 + 41844 0.71 0.561703
Target:  5'- aGCCAUCAGgauuucaGUGGACUCGUgcgccGAGu -3'
miRNA:   3'- -UGGUAGUCaacg---CGCCUGAGCAa----CUC- -5'
22555 5' -52.9 NC_005091.1 + 30510 0.67 0.790085
Target:  5'- gACCAUCAGcgGCGCGcuCUgCGUcaUGAGc -3'
miRNA:   3'- -UGGUAGUCaaCGCGCcuGA-GCA--ACUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.