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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22556 | 3' | -54.1 | NC_005091.1 | + | 21066 | 0.66 | 0.813798 |
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Target: 5'- --uCGGGUCagCUUCGAUGG-CUGCu -3' miRNA: 3'- guuGCCCAGaaGGGGCUAUCaGACGc -5' |
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| 22556 | 3' | -54.1 | NC_005091.1 | + | 14890 | 0.73 | 0.416793 |
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Target: 5'- gCGGCGGGUCUcgUCCCCGAUccggguaucGUCgcugGCa -3' miRNA: 3'- -GUUGCCCAGA--AGGGGCUAu--------CAGa---CGc -5' |
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| 22556 | 3' | -54.1 | NC_005091.1 | + | 15010 | 0.73 | 0.416793 |
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Target: 5'- gCGGCGGGUCUcgUCCCCGAUccggguaucGUCgcugGCa -3' miRNA: 3'- -GUUGCCCAGA--AGGGGCUAu--------CAGa---CGc -5' |
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| 22556 | 3' | -54.1 | NC_005091.1 | + | 15130 | 0.73 | 0.416793 |
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Target: 5'- gCGGCGGGUCUcgUCCCCGAUccggguaucGUCgcugGCa -3' miRNA: 3'- -GUUGCCCAGA--AGGGGCUAu--------CAGa---CGc -5' |
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| 22556 | 3' | -54.1 | NC_005091.1 | + | 2724 | 1.08 | 0.001673 |
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Target: 5'- aCAACGGGUCUUCCCCGAUAGUCUGCGc -3' miRNA: 3'- -GUUGCCCAGAAGGGGCUAUCAGACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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