Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22557 | 5' | -57.9 | NC_005091.1 | + | 33427 | 0.66 | 0.614301 |
Target: 5'- cGGACGCGaCGc-AGCAACCGgcauUGCCg -3' miRNA: 3'- cCCUGUGCgGCcuUCGUUGGC----ACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 49045 | 0.66 | 0.614301 |
Target: 5'- cGGGCGCGCUGcu-GCGcACCGUGUg- -3' miRNA: 3'- cCCUGUGCGGCcuuCGU-UGGCACGga -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 14492 | 0.66 | 0.611081 |
Target: 5'- aGGGCugcugaacgucgguGCGCCGGuAAGCGuGCCGcucgaUGCCg -3' miRNA: 3'- cCCUG--------------UGCGGCC-UUCGU-UGGC-----ACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 11481 | 0.66 | 0.603576 |
Target: 5'- aGGGGCAgGCCGaagcgcagcguGAcGCGGCCGacGCCc -3' miRNA: 3'- -CCCUGUgCGGC-----------CUuCGUUGGCa-CGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 23419 | 0.67 | 0.560991 |
Target: 5'- cGGGACGugggGCCuuGgcGCuuCCGUGCCUg -3' miRNA: 3'- -CCCUGUg---CGGc-CuuCGuuGGCACGGA- -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 44028 | 0.67 | 0.550462 |
Target: 5'- aGGu--CGCCgGGAAGCGACUGcGCCc -3' miRNA: 3'- cCCuguGCGG-CCUUCGUUGGCaCGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 4219 | 0.67 | 0.539995 |
Target: 5'- cGGcGGCGCGCCc---GUcACCGUGCCg -3' miRNA: 3'- -CC-CUGUGCGGccuuCGuUGGCACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 28267 | 0.67 | 0.529595 |
Target: 5'- cGGcGACGCGCUGGcGGCuugCGUGaCCUu -3' miRNA: 3'- -CC-CUGUGCGGCCuUCGuugGCAC-GGA- -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 16607 | 0.67 | 0.519271 |
Target: 5'- cGGGAaugccucccUGCGCCGccuGCcaAGCCGUGCCa -3' miRNA: 3'- -CCCU---------GUGCGGCcuuCG--UUGGCACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 18986 | 0.68 | 0.498868 |
Target: 5'- -cGACugGCCGcGAugcucGGCGgaAUCGUGCCg -3' miRNA: 3'- ccCUGugCGGC-CU-----UCGU--UGGCACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 44438 | 0.68 | 0.47883 |
Target: 5'- cGGGaACGCuuGCCGGu-GCAcccGCCaGUGCCg -3' miRNA: 3'- -CCC-UGUG--CGGCCuuCGU---UGG-CACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 10245 | 0.68 | 0.459195 |
Target: 5'- aGGACACGCgcacaucaGGAAGUgcggcgcucGGCgCGUGCCg -3' miRNA: 3'- cCCUGUGCGg-------CCUUCG---------UUG-GCACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 49551 | 0.68 | 0.459195 |
Target: 5'- cGGcCGCGUCGGggGCGucuUCGUaccGCCUg -3' miRNA: 3'- cCCuGUGCGGCCuuCGUu--GGCA---CGGA- -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 6854 | 0.68 | 0.44954 |
Target: 5'- cGGAuuuucgucCACGaacaCCGGGAcuuccGCGACCGUGCCg -3' miRNA: 3'- cCCU--------GUGC----GGCCUU-----CGUUGGCACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 26431 | 0.68 | 0.44954 |
Target: 5'- cGGGC-CGCCGGGgcugccaucguAGCGGCCGggcgcgagcagGCCg -3' miRNA: 3'- cCCUGuGCGGCCU-----------UCGUUGGCa----------CGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 14318 | 0.69 | 0.421264 |
Target: 5'- -cGACACGCaaGGcGGCucGACCGUGCCc -3' miRNA: 3'- ccCUGUGCGg-CCuUCG--UUGGCACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 19551 | 0.69 | 0.403021 |
Target: 5'- aGGGCACGCCGcAGGCcgUCGUGUUc -3' miRNA: 3'- cCCUGUGCGGCcUUCGuuGGCACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 37235 | 0.7 | 0.374038 |
Target: 5'- --aACACGCCGGGcgcguacgugGGCGauugguucaacgagACCGUGCCg -3' miRNA: 3'- cccUGUGCGGCCU----------UCGU--------------UGGCACGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 52452 | 0.7 | 0.359669 |
Target: 5'- aGGGCGCGCCGGGcGGCGACC---CCa -3' miRNA: 3'- cCCUGUGCGGCCU-UCGUUGGcacGGa -5' |
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22557 | 5' | -57.9 | NC_005091.1 | + | 53659 | 0.7 | 0.359669 |
Target: 5'- -cGGCGCGCCGGGguugcgcuGGCAAUgGUGCg- -3' miRNA: 3'- ccCUGUGCGGCCU--------UCGUUGgCACGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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