Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22559 | 5' | -62.7 | NC_005091.1 | + | 6508 | 0.66 | 0.390696 |
Target: 5'- uGCCGCCCgacucgauccacaucGUGcCugGCCucaauCCGCGcuuuuGGGGc -3' miRNA: 3'- -CGGCGGG---------------UACaGugCGG-----GGCGU-----CCCC- -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 1077 | 0.66 | 0.387304 |
Target: 5'- aGCgCGCCCGcGUCGagcagGUCCUGCGGGacGGa -3' miRNA: 3'- -CG-GCGGGUaCAGUg----CGGGGCGUCC--CC- -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 48849 | 0.66 | 0.370639 |
Target: 5'- aUUGCCCGUGUCACGgCCUGCcgcguGuGGc -3' miRNA: 3'- cGGCGGGUACAGUGCgGGGCGu----C-CCc -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 52309 | 0.66 | 0.370639 |
Target: 5'- aGCCGC---UGUCAaagcCGCCCCGCAcuaccGGGu -3' miRNA: 3'- -CGGCGgguACAGU----GCGGGGCGU-----CCCc -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 52526 | 0.66 | 0.35447 |
Target: 5'- cGCCGCCCGg--CGCGCCCUGauuccacuGGa- -3' miRNA: 3'- -CGGCGGGUacaGUGCGGGGCgu------CCcc -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 49302 | 0.67 | 0.331162 |
Target: 5'- aGCaGCCCA--UCGCGUgCUGaCGGGGGa -3' miRNA: 3'- -CGgCGGGUacAGUGCGgGGC-GUCCCC- -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 49471 | 0.68 | 0.301872 |
Target: 5'- uGCCGCgCGUGUucagCGCgaGCCCCgGCAGGa- -3' miRNA: 3'- -CGGCGgGUACA----GUG--CGGGG-CGUCCcc -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 14876 | 0.68 | 0.274619 |
Target: 5'- aGUCGCCCAUGUUGCaucCUCgCGCAGGa- -3' miRNA: 3'- -CGGCGGGUACAGUGc--GGG-GCGUCCcc -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 14995 | 0.68 | 0.274619 |
Target: 5'- aGUCGCCCAUGUUGCaucCUCgCGCAGGa- -3' miRNA: 3'- -CGGCGGGUACAGUGc--GGG-GCGUCCcc -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 9328 | 0.69 | 0.243368 |
Target: 5'- cGCCGgCCG-GUCGCcCUUCGCAGGGc -3' miRNA: 3'- -CGGCgGGUaCAGUGcGGGGCGUCCCc -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 16728 | 0.69 | 0.236902 |
Target: 5'- gGCCGCuacgucguccCCGUGaUugGCCCacgcuccgguaggCGCGGGGGa -3' miRNA: 3'- -CGGCG----------GGUACaGugCGGG-------------GCGUCCCC- -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 40963 | 0.7 | 0.214596 |
Target: 5'- cCCGCCCAgcgcugcgugcgcUG-CGCGCCuCCGaacugaAGGGGa -3' miRNA: 3'- cGGCGGGU-------------ACaGUGCGG-GGCg-----UCCCC- -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 16210 | 0.71 | 0.180357 |
Target: 5'- -gCGCCCGccGUCGCGCCCgGauCAGGGa -3' miRNA: 3'- cgGCGGGUa-CAGUGCGGGgC--GUCCCc -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 21705 | 0.72 | 0.15461 |
Target: 5'- gGCCGCCCGccgGUCGaucCG-CuuGCGGGGGa -3' miRNA: 3'- -CGGCGGGUa--CAGU---GCgGggCGUCCCC- -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 6214 | 0.74 | 0.112587 |
Target: 5'- gGCCGCCCAcuucgcuUGaCugGCUCCuagcGCGGGGGc -3' miRNA: 3'- -CGGCGGGU-------ACaGugCGGGG----CGUCCCC- -5' |
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22559 | 5' | -62.7 | NC_005091.1 | + | 5178 | 1.1 | 0.0002 |
Target: 5'- gGCCGCCCAUGUCACGCCCCGCAGGGGc -3' miRNA: 3'- -CGGCGGGUACAGUGCGGGGCGUCCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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