Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22560 | 3' | -54.5 | NC_005091.1 | + | 13822 | 0.66 | 0.796099 |
Target: 5'- aCUUCGGC-UCGG-GCGGuuCGcCUGCg -3' miRNA: 3'- aGAGGCUGaAGCCgCGCUu-GCaGACG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 7572 | 0.66 | 0.776553 |
Target: 5'- uUCUUCGACUUa--CGCGAGCGcgUGCu -3' miRNA: 3'- -AGAGGCUGAAgccGCGCUUGCagACG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 983 | 0.66 | 0.776553 |
Target: 5'- aUCUCCGucccgcaggccuGC-UCGaCGCGGGCGcgCUGCu -3' miRNA: 3'- -AGAGGC------------UGaAGCcGCGCUUGCa-GACG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 46092 | 0.67 | 0.746162 |
Target: 5'- --aCCGAUaUCGGCGaaGACGagCUGCa -3' miRNA: 3'- agaGGCUGaAGCCGCgcUUGCa-GACG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 42663 | 0.67 | 0.725315 |
Target: 5'- -gUCCGAgCgugcgCGGCGCGGacaaGCGUCUucucgGCg -3' miRNA: 3'- agAGGCU-Gaa---GCCGCGCU----UGCAGA-----CG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 18309 | 0.68 | 0.704103 |
Target: 5'- gUUUCCGACgUUCuGcGCGCGGAC--CUGCu -3' miRNA: 3'- -AGAGGCUG-AAG-C-CGCGCUUGcaGACG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 32398 | 0.68 | 0.693389 |
Target: 5'- cUCUCCaugugGACUUCGGUuuCGGAUGUCgaGCc -3' miRNA: 3'- -AGAGG-----CUGAAGCCGc-GCUUGCAGa-CG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 2386 | 0.68 | 0.682616 |
Target: 5'- cCUUCGGCgUCGuCGCGAuGCGUCgugGCu -3' miRNA: 3'- aGAGGCUGaAGCcGCGCU-UGCAGa--CG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 29605 | 0.68 | 0.671796 |
Target: 5'- cUCgCCGAUcUCGGUGCGGGCGa--GCg -3' miRNA: 3'- -AGaGGCUGaAGCCGCGCUUGCagaCG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 48592 | 0.68 | 0.660939 |
Target: 5'- -aUCCGGCga-GGCGCGAAUGgcgggaggGCg -3' miRNA: 3'- agAGGCUGaagCCGCGCUUGCaga-----CG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 32247 | 0.68 | 0.660939 |
Target: 5'- aUCUCgCGugUUCaGUGCGauGugGUUUGCc -3' miRNA: 3'- -AGAG-GCugAAGcCGCGC--UugCAGACG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 55827 | 0.68 | 0.660939 |
Target: 5'- cCUCCGuuUUCgguuucaggGGCGCGGGcCGUCaGCg -3' miRNA: 3'- aGAGGCugAAG---------CCGCGCUU-GCAGaCG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 6015 | 0.68 | 0.650056 |
Target: 5'- gUCUauguaCGAgggCGGCGCGGACGUUcggGCg -3' miRNA: 3'- -AGAg----GCUgaaGCCGCGCUUGCAGa--CG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 51994 | 0.68 | 0.650056 |
Target: 5'- gCUCgCGAUggUCGGCGCuacGGCGUC-GCa -3' miRNA: 3'- aGAG-GCUGa-AGCCGCGc--UUGCAGaCG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 23134 | 0.69 | 0.639157 |
Target: 5'- aUCUCCGAuccgaaCUUCGuGCccgauGCGAugGCGUCgGCg -3' miRNA: 3'- -AGAGGCU------GAAGC-CG-----CGCU--UGCAGaCG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 29078 | 0.69 | 0.617349 |
Target: 5'- --aCCGAgUgcaguUCGGCGCGGagACGcCUGCc -3' miRNA: 3'- agaGGCUgA-----AGCCGCGCU--UGCaGACG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 12112 | 0.69 | 0.617349 |
Target: 5'- aCUCCuGaACUggcCGGCcaucUGAACGUCUGCg -3' miRNA: 3'- aGAGG-C-UGAa--GCCGc---GCUUGCAGACG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 49026 | 0.7 | 0.584755 |
Target: 5'- aUUCUGACgugCGGUgcuGCGGGCGcgCUGCu -3' miRNA: 3'- aGAGGCUGaa-GCCG---CGCUUGCa-GACG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 29992 | 0.7 | 0.584755 |
Target: 5'- aUCUUCGugUggaagcgagCGGUcaGCG-GCGUCUGCg -3' miRNA: 3'- -AGAGGCugAa--------GCCG--CGCuUGCAGACG- -5' |
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22560 | 3' | -54.5 | NC_005091.1 | + | 14072 | 0.7 | 0.573957 |
Target: 5'- aUCUCCGGaacgggCGGUGCGAcgacgguaucGCGUaucaCUGCg -3' miRNA: 3'- -AGAGGCUgaa---GCCGCGCU----------UGCA----GACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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