Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22561 | 3' | -54.9 | NC_005091.1 | + | 51269 | 0.66 | 0.759673 |
Target: 5'- uCGCCGUCGcgCccGUGGGCGAu--- -3' miRNA: 3'- uGCGGCAGCuaGaaCGCUCGCUucgu -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 10203 | 0.66 | 0.759673 |
Target: 5'- gGCGUaaCGUCcGUCUcGCGcGCGAGGUAg -3' miRNA: 3'- -UGCG--GCAGcUAGAaCGCuCGCUUCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 24573 | 0.66 | 0.759673 |
Target: 5'- aGCGgUGUCGAUCgggGCG-GCGucguuGCAa -3' miRNA: 3'- -UGCgGCAGCUAGaa-CGCuCGCuu---CGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 31736 | 0.66 | 0.759673 |
Target: 5'- aAUGCCGUaCGccuUC-UGCGAGCcguccGAGGCGg -3' miRNA: 3'- -UGCGGCA-GCu--AGaACGCUCG-----CUUCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 51865 | 0.66 | 0.753486 |
Target: 5'- gACGCCGggaaGAUCgcggaUGCGAugcugcgcgagucgcGCGAAGUg -3' miRNA: 3'- -UGCGGCag--CUAGa----ACGCU---------------CGCUUCGu -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 32662 | 0.66 | 0.728313 |
Target: 5'- uUGCCGUCGAcCUUGUGGuCGccGGCAu -3' miRNA: 3'- uGCGGCAGCUaGAACGCUcGCu-UCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 379 | 0.66 | 0.728313 |
Target: 5'- gACGCCGUCGugaagGCG-GCcAAGCGg -3' miRNA: 3'- -UGCGGCAGCuagaaCGCuCGcUUCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 57773 | 0.66 | 0.728313 |
Target: 5'- gACGCCGUCGugaagGCG-GCcAAGCGg -3' miRNA: 3'- -UGCGGCAGCuagaaCGCuCGcUUCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 39982 | 0.67 | 0.674266 |
Target: 5'- cGCGUCGcCGAUCUUGCGcagGGUGAc--- -3' miRNA: 3'- -UGCGGCaGCUAGAACGC---UCGCUucgu -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 54101 | 0.67 | 0.663293 |
Target: 5'- uGCGCCGUCGcgCUggaaaaGCGAGaCGccGGCc -3' miRNA: 3'- -UGCGGCAGCuaGAa-----CGCUC-GCu-UCGu -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 32029 | 0.67 | 0.663293 |
Target: 5'- gGC-CCGUCGc-CUUgGCGAGCGAcgcgAGCAg -3' miRNA: 3'- -UGcGGCAGCuaGAA-CGCUCGCU----UCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 23482 | 0.67 | 0.65229 |
Target: 5'- gGCGCUGcUCGGUCUguucgGCG-GCGcuGCGu -3' miRNA: 3'- -UGCGGC-AGCUAGAa----CGCuCGCuuCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 18490 | 0.68 | 0.630238 |
Target: 5'- aGCGUCagcuucgCGGUCUUGCGGuCGAAGUAg -3' miRNA: 3'- -UGCGGca-----GCUAGAACGCUcGCUUCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 29874 | 0.68 | 0.61921 |
Target: 5'- -aGCCGUCGcgCUgcaaugugaUGcCGAGUGAAGUu -3' miRNA: 3'- ugCGGCAGCuaGA---------AC-GCUCGCUUCGu -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 49049 | 0.69 | 0.564422 |
Target: 5'- cGCGCUGcUGcgCaccgugUGCGGGCGggGCGa -3' miRNA: 3'- -UGCGGCaGCuaGa-----ACGCUCGCuuCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 16066 | 0.69 | 0.564422 |
Target: 5'- -aGCCGUCGcAUCgca-GAGCGAcaAGCAg -3' miRNA: 3'- ugCGGCAGC-UAGaacgCUCGCU--UCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 29602 | 0.7 | 0.500546 |
Target: 5'- uUGCuCGcCGAUCUcggUGCGGGCG-AGCGg -3' miRNA: 3'- uGCG-GCaGCUAGA---ACGCUCGCuUCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 5694 | 0.7 | 0.47995 |
Target: 5'- uGCGCCGgaucaucccggCGAUCUccuuaGCGAGCGcAGCu -3' miRNA: 3'- -UGCGGCa----------GCUAGAa----CGCUCGCuUCGu -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 21699 | 0.71 | 0.449861 |
Target: 5'- cCGCCgGUCGAUCcgcUUGCGGG-GGAGUAu -3' miRNA: 3'- uGCGG-CAGCUAG---AACGCUCgCUUCGU- -5' |
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22561 | 3' | -54.9 | NC_005091.1 | + | 4831 | 0.72 | 0.402131 |
Target: 5'- cGCGgCGgcaaCGGUCaaGCGGGCGAAGCc -3' miRNA: 3'- -UGCgGCa---GCUAGaaCGCUCGCUUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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