Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22562 | 3' | -56.3 | NC_005091.1 | + | 41138 | 0.66 | 0.695235 |
Target: 5'- aGUCuGCAcGCACGUCGGUCGg----- -3' miRNA: 3'- cCAG-CGUaCGUGCAGCCGGCaaggau -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 11427 | 0.66 | 0.684479 |
Target: 5'- -uUCGCGgcgGcCACGUCGGCCGacgcuuucgCCUu -3' miRNA: 3'- ccAGCGUa--C-GUGCAGCCGGCaa-------GGAu -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 55439 | 0.66 | 0.684479 |
Target: 5'- cGUCGCGgccGC-CGUCaGCCGggcgUCCUc -3' miRNA: 3'- cCAGCGUa--CGuGCAGcCGGCa---AGGAu -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 41882 | 0.66 | 0.673674 |
Target: 5'- uGUCGaggccGCACGUCGGCaggUCCUc -3' miRNA: 3'- cCAGCgua--CGUGCAGCCGgcaAGGAu -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 29528 | 0.66 | 0.651959 |
Target: 5'- aGGUCGU-UGCGCGUC-GCC-UUCUUGg -3' miRNA: 3'- -CCAGCGuACGUGCAGcCGGcAAGGAU- -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 46321 | 0.68 | 0.565141 |
Target: 5'- -uUCGCGUcGUAguUGUCGGCCGUcUCCg- -3' miRNA: 3'- ccAGCGUA-CGU--GCAGCCGGCA-AGGau -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 12083 | 0.69 | 0.512316 |
Target: 5'- aGGUUGCGUaCGCG-CGaGCCGggCCUGc -3' miRNA: 3'- -CCAGCGUAcGUGCaGC-CGGCaaGGAU- -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 45569 | 0.69 | 0.491748 |
Target: 5'- gGGUCGCcgAUGUgaagcuggccgGCGUCGGCU--UCCUGa -3' miRNA: 3'- -CCAGCG--UACG-----------UGCAGCCGGcaAGGAU- -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 49727 | 0.7 | 0.451819 |
Target: 5'- --aUGCGggGCACGUCGGCCGUgCgUGa -3' miRNA: 3'- ccaGCGUa-CGUGCAGCCGGCAaGgAU- -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 15256 | 0.7 | 0.442115 |
Target: 5'- --gCGCGUGUAcCGUUGGCCG-UCCg- -3' miRNA: 3'- ccaGCGUACGU-GCAGCCGGCaAGGau -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 51491 | 0.71 | 0.40452 |
Target: 5'- cGGaUCGCAUGCACG--GGCCaGUUCUa- -3' miRNA: 3'- -CC-AGCGUACGUGCagCCGG-CAAGGau -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 518 | 0.71 | 0.386494 |
Target: 5'- --aCGCAgGCACG-CGGCCGggaaUCCUGa -3' miRNA: 3'- ccaGCGUaCGUGCaGCCGGCa---AGGAU- -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 57911 | 0.71 | 0.386494 |
Target: 5'- --aCGCAgGCACG-CGGCCGggaaUCCUGa -3' miRNA: 3'- ccaGCGUaCGUGCaGCCGGCa---AGGAU- -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 4302 | 0.74 | 0.255662 |
Target: 5'- uGGUCGUGUuCGCGcCGGCCGUcgucuUCCUGu -3' miRNA: 3'- -CCAGCGUAcGUGCaGCCGGCA-----AGGAU- -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 57423 | 0.78 | 0.134995 |
Target: 5'- --gCGCAUGCGCGagUCGGgCGUUCCUAu -3' miRNA: 3'- ccaGCGUACGUGC--AGCCgGCAAGGAU- -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 29 | 0.78 | 0.134995 |
Target: 5'- --gCGCAUGCGCGagUCGGgCGUUCCUAu -3' miRNA: 3'- ccaGCGUACGUGC--AGCCgGCAAGGAU- -5' |
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22562 | 3' | -56.3 | NC_005091.1 | + | 8180 | 1.08 | 0.00097 |
Target: 5'- gGGUCGCAUGCACGUCGGCCGUUCCUAc -3' miRNA: 3'- -CCAGCGUACGUGCAGCCGGCAAGGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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