Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22562 | 5' | -53.7 | NC_005091.1 | + | 48643 | 0.66 | 0.806334 |
Target: 5'- gGCUgGCGcGCAGgCCCGaUGAgGAucGCUCg -3' miRNA: 3'- -CGA-UGCaCGUCgGGGC-ACUaCU--UGAG- -5' |
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22562 | 5' | -53.7 | NC_005091.1 | + | 35198 | 0.66 | 0.786716 |
Target: 5'- aGCUGCugaccgGCcgcucGGCCCCGUGAucgUGGAC-Ca -3' miRNA: 3'- -CGAUGca----CG-----UCGGGGCACU---ACUUGaG- -5' |
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22562 | 5' | -53.7 | NC_005091.1 | + | 49199 | 0.67 | 0.73499 |
Target: 5'- uGCggaugACGUGCGGCuucuccCCCGUcagcacgcGAUGGGCUg -3' miRNA: 3'- -CGa----UGCACGUCG------GGGCA--------CUACUUGAg -5' |
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22562 | 5' | -53.7 | NC_005091.1 | + | 31399 | 0.67 | 0.73499 |
Target: 5'- --aACGUGCaaGGCCgCGUGAUcgcuGCUCg -3' miRNA: 3'- cgaUGCACG--UCGGgGCACUAcu--UGAG- -5' |
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22562 | 5' | -53.7 | NC_005091.1 | + | 16731 | 0.67 | 0.713475 |
Target: 5'- cGCUACGU-CGuCCCCGUGAUuGGcccacGCUCc -3' miRNA: 3'- -CGAUGCAcGUcGGGGCACUA-CU-----UGAG- -5' |
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22562 | 5' | -53.7 | NC_005091.1 | + | 26348 | 0.68 | 0.702589 |
Target: 5'- cGCUACGaugGCAGCCCCG-GcgGcccGCg- -3' miRNA: 3'- -CGAUGCa--CGUCGGGGCaCuaCu--UGag -5' |
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22562 | 5' | -53.7 | NC_005091.1 | + | 41959 | 0.68 | 0.691632 |
Target: 5'- uGCcgACGUGCGGCCUCGacAUuuACUCg -3' miRNA: 3'- -CGa-UGCACGUCGGGGCacUAcuUGAG- -5' |
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22562 | 5' | -53.7 | NC_005091.1 | + | 1155 | 0.69 | 0.602795 |
Target: 5'- cGCUaacugGCGUGCAGCcauCCCGgcgaaGAUGGAUUUc -3' miRNA: 3'- -CGA-----UGCACGUCG---GGGCa----CUACUUGAG- -5' |
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22562 | 5' | -53.7 | NC_005091.1 | + | 11495 | 0.69 | 0.602795 |
Target: 5'- cGCUACGauaGCAGCCCCGauagccgcGAUGA--UCg -3' miRNA: 3'- -CGAUGCa--CGUCGGGGCa-------CUACUugAG- -5' |
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22562 | 5' | -53.7 | NC_005091.1 | + | 51503 | 0.7 | 0.568529 |
Target: 5'- cGCUgcGCGUGCAGUCgcgauucaagcugCCGUaGAcGAACUCg -3' miRNA: 3'- -CGA--UGCACGUCGG-------------GGCA-CUaCUUGAG- -5' |
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22562 | 5' | -53.7 | NC_005091.1 | + | 19185 | 0.72 | 0.453493 |
Target: 5'- uGCgGCGcuuUGCcguGGCCCCGUcGAUGAACUg -3' miRNA: 3'- -CGaUGC---ACG---UCGGGGCA-CUACUUGAg -5' |
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22562 | 5' | -53.7 | NC_005091.1 | + | 8146 | 1.11 | 0.001013 |
Target: 5'- cGCUACGUGCAGCCCCGUGAUGAACUCc -3' miRNA: 3'- -CGAUGCACGUCGGGGCACUACUUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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