Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22563 | 3' | -52 | NC_005091.1 | + | 16157 | 0.66 | 0.895914 |
Target: 5'- --cGGCCGGCAcuacgaagUAUCU-CCGGGa -3' miRNA: 3'- cauCUGGCCGUauaa----GUAGAcGGCCC- -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 57642 | 0.66 | 0.893747 |
Target: 5'- -gAGACCGGCugccgggUCAacuUCUuucggcgaauccggGCCGGGc -3' miRNA: 3'- caUCUGGCCGuaua---AGU---AGA--------------CGGCCC- -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 33885 | 0.66 | 0.884076 |
Target: 5'- --cGcCCGGCGUacccaucggcuucagGUUCAgcuUCgUGCCGGGa -3' miRNA: 3'- cauCuGGCCGUA---------------UAAGU---AG-ACGGCCC- -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 18243 | 0.66 | 0.865041 |
Target: 5'- -gAGGUCGGCAg--UCGggaUUGCCGGGu -3' miRNA: 3'- caUCUGGCCGUauaAGUa--GACGGCCC- -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 41241 | 0.67 | 0.826463 |
Target: 5'- -aAGGCCGGUgaucgcgcgCAUCUGCCGa- -3' miRNA: 3'- caUCUGGCCGuauaa----GUAGACGGCcc -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 18426 | 0.67 | 0.824606 |
Target: 5'- -cAGGCCGGaCGUGUagucgaaaugcggaaUUGUC-GCCGGGu -3' miRNA: 3'- caUCUGGCC-GUAUA---------------AGUAGaCGGCCC- -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 7206 | 0.67 | 0.819926 |
Target: 5'- --cGGCCGGCAgauggcggcgcguUAccUUCGUCuUGCCGGc -3' miRNA: 3'- cauCUGGCCGU-------------AU--AAGUAG-ACGGCCc -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 33218 | 0.68 | 0.801685 |
Target: 5'- -gAGGCCGGCGUAaugcgcggcgacCAUCUGCCcgcgcagguugaaccGGGu -3' miRNA: 3'- caUCUGGCCGUAUaa----------GUAGACGG---------------CCC- -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 1307 | 0.68 | 0.791813 |
Target: 5'- ---aGCCGGCcgaggaaguccAUGUUCAUCaugGUCGGGa -3' miRNA: 3'- caucUGGCCG-----------UAUAAGUAGa--CGGCCC- -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 2505 | 0.69 | 0.760163 |
Target: 5'- -gAGAUCGGCGUGUuucUCAaucacguUCUGCCGc- -3' miRNA: 3'- caUCUGGCCGUAUA---AGU-------AGACGGCcc -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 51095 | 0.69 | 0.729365 |
Target: 5'- gGUAaACCGGCAUAacugCAcCgUGCCGGGc -3' miRNA: 3'- -CAUcUGGCCGUAUaa--GUaG-ACGGCCC- -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 56448 | 0.71 | 0.640885 |
Target: 5'- --cGGCUGGCGUAUggggCuUCUGgCGGGa -3' miRNA: 3'- cauCUGGCCGUAUAa---GuAGACgGCCC- -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 10015 | 0.74 | 0.481183 |
Target: 5'- cUGGAacCCGGCAcgacggcgaccacguUGUUCAUC-GCCGGGc -3' miRNA: 3'- cAUCU--GGCCGU---------------AUAAGUAGaCGGCCC- -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 44230 | 0.77 | 0.320272 |
Target: 5'- -aAGACCGGCAUAcgccccgaUCAUCUGCuCGGc -3' miRNA: 3'- caUCUGGCCGUAUa-------AGUAGACG-GCCc -5' |
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22563 | 3' | -52 | NC_005091.1 | + | 8371 | 1.1 | 0.001859 |
Target: 5'- cGUAGACCGGCAUAUUCAUCUGCCGGGc -3' miRNA: 3'- -CAUCUGGCCGUAUAAGUAGACGGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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