Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22565 | 3' | -59.2 | NC_005091.1 | + | 32167 | 0.66 | 0.533382 |
Target: 5'- --cGGCcGGCGGCaaGGGCAAcgaaGCUGa -3' miRNA: 3'- gcaCCGaCCGCCG--UCCGUUaug-CGGC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 14460 | 0.66 | 0.533382 |
Target: 5'- uCGUcGGCUguccgGGUcaGGCGGGCAAcuacgcgACGCCu -3' miRNA: 3'- -GCA-CCGA-----CCG--CCGUCCGUUa------UGCGGc -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 37192 | 0.66 | 0.512784 |
Target: 5'- -uUGGC-GGCGGCcuGCGAUACgGUCa -3' miRNA: 3'- gcACCGaCCGCCGucCGUUAUG-CGGc -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 44821 | 0.66 | 0.49252 |
Target: 5'- --gGGC-GGCGGCaAGGCAGcaacgaaGCCGa -3' miRNA: 3'- gcaCCGaCCGCCG-UCCGUUaug----CGGC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 20011 | 0.66 | 0.483522 |
Target: 5'- --cGGCguuaucacugccgugGGCGGCGauuGGUucgAGUGCGCCGa -3' miRNA: 3'- gcaCCGa--------------CCGCCGU---CCG---UUAUGCGGC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 52963 | 0.67 | 0.446446 |
Target: 5'- cCGUGGCUGGUuguaccugaucgcGGCcGGCugcaaacaaaucgucGGaGCGCCGa -3' miRNA: 3'- -GCACCGACCG-------------CCGuCCG---------------UUaUGCGGC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 49592 | 0.67 | 0.440737 |
Target: 5'- cCGUGGCUGGCGcguucugccugccGCucgucguguucGCAAUugGCCu -3' miRNA: 3'- -GCACCGACCGC-------------CGuc---------CGUUAugCGGc -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 50298 | 0.67 | 0.434129 |
Target: 5'- uCGUcGGCUcgcGGCcgaucacccGGCAGGUGAUAuCGCCa -3' miRNA: 3'- -GCA-CCGA---CCG---------CCGUCCGUUAU-GCGGc -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 23363 | 0.68 | 0.406476 |
Target: 5'- uCGUcGGUcagccgGGCGGCAugucGcGCAGUGCGUCGg -3' miRNA: 3'- -GCA-CCGa-----CCGCCGU----C-CGUUAUGCGGC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 16106 | 0.68 | 0.397508 |
Target: 5'- --cGGCcGGCGGCAG-CAcgACGCUu -3' miRNA: 3'- gcaCCGaCCGCCGUCcGUuaUGCGGc -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 33746 | 0.68 | 0.388667 |
Target: 5'- uGUGGCUGgaagaggcuaucGCGGCuGGUgaagucccGAUGcCGCCGg -3' miRNA: 3'- gCACCGAC------------CGCCGuCCG--------UUAU-GCGGC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 25246 | 0.68 | 0.379957 |
Target: 5'- --cGGCcuucgcuaUGGCGGCGGuCGGUGCGuCCGg -3' miRNA: 3'- gcaCCG--------ACCGCCGUCcGUUAUGC-GGC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 16237 | 0.68 | 0.379957 |
Target: 5'- aCGaGGCcGcCGGCAuGCGAUGCGCCGc -3' miRNA: 3'- -GCaCCGaCcGCCGUcCGUUAUGCGGC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 44884 | 0.68 | 0.371378 |
Target: 5'- --aGGCgguacgGGCGGC-GGCAAgGCgGCCGa -3' miRNA: 3'- gcaCCGa-----CCGCCGuCCGUUaUG-CGGC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 23316 | 0.69 | 0.35462 |
Target: 5'- aGUGcGCaaUGGCaGCAGGCAcggaaGCGCCa -3' miRNA: 3'- gCAC-CG--ACCGcCGUCCGUua---UGCGGc -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 40205 | 0.69 | 0.338403 |
Target: 5'- aGUGGCU-GCGGCAGGacu--CGCaCGg -3' miRNA: 3'- gCACCGAcCGCCGUCCguuauGCG-GC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 28982 | 0.69 | 0.322731 |
Target: 5'- --cGGCaGG-GGCAGGCGucuccGCGCCGa -3' miRNA: 3'- gcaCCGaCCgCCGUCCGUua---UGCGGC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 48645 | 0.71 | 0.272189 |
Target: 5'- -cUGGCUGGCGcGCAGGCccGAUGaggauCGCuCGg -3' miRNA: 3'- gcACCGACCGC-CGUCCG--UUAU-----GCG-GC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 26518 | 0.74 | 0.16684 |
Target: 5'- uCGUcaGGCUGGcCGuGCGGGC-AUugGCCGc -3' miRNA: 3'- -GCA--CCGACC-GC-CGUCCGuUAugCGGC- -5' |
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22565 | 3' | -59.2 | NC_005091.1 | + | 23107 | 0.79 | 0.069254 |
Target: 5'- gCGaUGGCgucGGCGGCAGGCGAUAcCGUCa -3' miRNA: 3'- -GC-ACCGa--CCGCCGUCCGUUAU-GCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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