Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22567 | 3' | -54.2 | NC_005091.1 | + | 10959 | 0.66 | 0.795531 |
Target: 5'- cCGCCGGguCGAGCUGUuucccgaGGU-CGAUCAu -3' miRNA: 3'- -GCGGCCauGUUCGACG-------CCAaGUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 16985 | 0.66 | 0.790615 |
Target: 5'- gGCUGGgcggacggagcguCGGGCUGgGGgaCGACCAc -3' miRNA: 3'- gCGGCCau-----------GUUCGACgCCaaGUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 29606 | 0.66 | 0.786652 |
Target: 5'- uCGCCgaucucGGUGCGGGCgaGCGGgagGGCCGu -3' miRNA: 3'- -GCGG------CCAUGUUCGa-CGCCaagUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 1321 | 0.66 | 0.776633 |
Target: 5'- aCGCCGGgugACGGGCaGCGcacCGACUAc -3' miRNA: 3'- -GCGGCCa--UGUUCGaCGCcaaGUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 18139 | 0.66 | 0.766462 |
Target: 5'- aCGUCGGUcggcGCGAGCUucGCGGcccggCGAUCGa -3' miRNA: 3'- -GCGGCCA----UGUUCGA--CGCCaa---GUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 5208 | 0.66 | 0.766462 |
Target: 5'- gCGCaCGGUGCAAGgaGUGcaaCGGCCGc -3' miRNA: 3'- -GCG-GCCAUGUUCgaCGCcaaGUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 53080 | 0.67 | 0.735153 |
Target: 5'- cCGuuGGUcGCGAGCauUGCGG--CGACCGu -3' miRNA: 3'- -GCggCCA-UGUUCG--ACGCCaaGUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 28961 | 0.67 | 0.735153 |
Target: 5'- uGCUucuGGUGCAgGGCUG-GG-UCAACCAu -3' miRNA: 3'- gCGG---CCAUGU-UCGACgCCaAGUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 56897 | 0.67 | 0.735153 |
Target: 5'- uGCCGucGCuGGCUGCGcGgugCAGCCAc -3' miRNA: 3'- gCGGCcaUGuUCGACGC-Caa-GUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 33127 | 0.67 | 0.72449 |
Target: 5'- aCGCCuGUGCGAGUUGCGuGcgCugGACCu -3' miRNA: 3'- -GCGGcCAUGUUCGACGC-CaaG--UUGGu -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 11393 | 0.67 | 0.713735 |
Target: 5'- gGCCGcGU-CAcGCUGCGcUUCGGCCu -3' miRNA: 3'- gCGGC-CAuGUuCGACGCcAAGUUGGu -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 6704 | 0.67 | 0.702897 |
Target: 5'- gGCCGGccaACAccgcuuGGCUGCGGU-CGccgucGCCAa -3' miRNA: 3'- gCGGCCa--UGU------UCGACGCCAaGU-----UGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 9364 | 0.67 | 0.702897 |
Target: 5'- aGCCGGUcgGCGgccAGCgGCGaGcagUCGACCAu -3' miRNA: 3'- gCGGCCA--UGU---UCGaCGC-Ca--AGUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 55847 | 0.68 | 0.657853 |
Target: 5'- aGCCGGUcagcaacuggcgcACGAGCUGCacGGccUGACCAu -3' miRNA: 3'- gCGGCCA-------------UGUUCGACG--CCaaGUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 15848 | 0.69 | 0.603541 |
Target: 5'- gCGUCGGcaugcguguUGcCGAGCUGCGGUUCA-UCGa -3' miRNA: 3'- -GCGGCC---------AU-GUUCGACGCCAAGUuGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 34402 | 0.69 | 0.592493 |
Target: 5'- uGCUGGUACGGGUucgacagGCGGUcggUCGauACCAu -3' miRNA: 3'- gCGGCCAUGUUCGa------CGCCA---AGU--UGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 30706 | 0.7 | 0.548728 |
Target: 5'- uCGCCGGUAUAGGUgucGUGGUaCGugUAg -3' miRNA: 3'- -GCGGCCAUGUUCGa--CGCCAaGUugGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 57151 | 0.71 | 0.464802 |
Target: 5'- uGCCGGU-CGAguucguGCUGCGGUccuUCAGCUc -3' miRNA: 3'- gCGGCCAuGUU------CGACGCCA---AGUUGGu -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 31538 | 0.72 | 0.448807 |
Target: 5'- uGCUGGcgcucguuacagcgACGAGCgUGCGGUaUCGACCAa -3' miRNA: 3'- gCGGCCa-------------UGUUCG-ACGCCA-AGUUGGU- -5' |
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22567 | 3' | -54.2 | NC_005091.1 | + | 44884 | 0.72 | 0.406459 |
Target: 5'- aGgCGGUACGGGCgGCGGcaaggCGGCCGa -3' miRNA: 3'- gCgGCCAUGUUCGaCGCCaa---GUUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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