Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22567 | 5' | -55.3 | NC_005091.1 | + | 27255 | 0.66 | 0.74242 |
Target: 5'- --gCGUC-GCGCCGCAGUccgagaUACCAc -3' miRNA: 3'- guaGCAGcCGCGGCGUCGaa----AUGGUc -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 10794 | 0.66 | 0.74242 |
Target: 5'- uCAUCGggugcuugcguUCGaGCGCCGUAGCgaaguCCAu -3' miRNA: 3'- -GUAGC-----------AGC-CGCGGCGUCGaaau-GGUc -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 26401 | 0.66 | 0.710585 |
Target: 5'- gGUCugcugCGGCaGCCGCAGCcugcgUUGCCu- -3' miRNA: 3'- gUAGca---GCCG-CGGCGUCGa----AAUGGuc -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 18230 | 0.66 | 0.699804 |
Target: 5'- gAUCGcCGG-GCCGCgaAGCUcgGCCGa -3' miRNA: 3'- gUAGCaGCCgCGGCG--UCGAaaUGGUc -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 19629 | 0.66 | 0.688957 |
Target: 5'- uGUCGgCGGCGCucggcccgaaagCGCAGCa--ACCGGa -3' miRNA: 3'- gUAGCaGCCGCG------------GCGUCGaaaUGGUC- -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 23466 | 0.66 | 0.688957 |
Target: 5'- -uUCGgCGGCGCUGCgucGGCUgccCCGGc -3' miRNA: 3'- guAGCaGCCGCGGCG---UCGAaauGGUC- -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 26781 | 0.67 | 0.678055 |
Target: 5'- cCGUCGagGGCGCgGCcaucGCg-UACCAGa -3' miRNA: 3'- -GUAGCagCCGCGgCGu---CGaaAUGGUC- -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 52844 | 0.68 | 0.586865 |
Target: 5'- cCAUCGuUCGGCGCUccgacgauuuguuuGCAGCcg-GCCGc -3' miRNA: 3'- -GUAGC-AGCCGCGG--------------CGUCGaaaUGGUc -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 36870 | 0.69 | 0.557506 |
Target: 5'- uGUUGUCGGUGUCGCgcgAGCgc-GCCAu -3' miRNA: 3'- gUAGCAGCCGCGGCG---UCGaaaUGGUc -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 35227 | 0.69 | 0.545661 |
Target: 5'- -cUCGcggCGGCGCCGCgacgcguaccacaAGCUgcugACCGGc -3' miRNA: 3'- guAGCa--GCCGCGGCG-------------UCGAaa--UGGUC- -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 50295 | 0.69 | 0.53603 |
Target: 5'- cCAUCGUCGGC-UCGCGGCcg-AUCAc -3' miRNA: 3'- -GUAGCAGCCGcGGCGUCGaaaUGGUc -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 34771 | 0.7 | 0.494019 |
Target: 5'- gCAUCGUgGGCG-CGCAGU---ACCGGc -3' miRNA: 3'- -GUAGCAgCCGCgGCGUCGaaaUGGUC- -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 24950 | 0.7 | 0.463522 |
Target: 5'- --aCGauUCGGcCGCCGCAGUgccgACCAGu -3' miRNA: 3'- guaGC--AGCC-GCGGCGUCGaaa-UGGUC- -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 17408 | 0.7 | 0.452588 |
Target: 5'- -cUCGUCGGCGCUGCGuucgccuGCaaUACCGa -3' miRNA: 3'- guAGCAGCCGCGGCGU-------CGaaAUGGUc -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 14265 | 0.71 | 0.434039 |
Target: 5'- uGUCGUCGGCGa-GCGGCcagUACCu- -3' miRNA: 3'- gUAGCAGCCGCggCGUCGaa-AUGGuc -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 49878 | 0.72 | 0.369723 |
Target: 5'- gCAUCGUCGGCcCCuGCGGUgcggUGCCGa -3' miRNA: 3'- -GUAGCAGCCGcGG-CGUCGaa--AUGGUc -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 2380 | 0.73 | 0.312239 |
Target: 5'- gCGUCGUCGcgauGCGUCGUGGCUucUUGCCAc -3' miRNA: 3'- -GUAGCAGC----CGCGGCGUCGA--AAUGGUc -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 13901 | 0.74 | 0.297111 |
Target: 5'- cCGUCGUCuGGUugaaCCGCAGCUUgUACCGGc -3' miRNA: 3'- -GUAGCAG-CCGc---GGCGUCGAA-AUGGUC- -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 35150 | 0.75 | 0.261797 |
Target: 5'- gCGUCG-CGGCGCCGCcGCgagguacUGCCGGc -3' miRNA: 3'- -GUAGCaGCCGCGGCGuCGaa-----AUGGUC- -5' |
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22567 | 5' | -55.3 | NC_005091.1 | + | 13955 | 1.09 | 0.001058 |
Target: 5'- gCAUCGUCGGCGCCGCAGCUUUACCAGa -3' miRNA: 3'- -GUAGCAGCCGCGGCGUCGAAAUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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